Biffani, Stefano

Bonelli, Antonio

Bozzi, Riccardo

Crovetti, Alessandro

Ghezzi, Silvia

English

Florence Research

[page 128]                                                             [Ital J Anim Sci vol.14:s1, 2015]                                      Italian Journal of Animal Science 2015; volume 14: supplement 1GENETICS AND GENOMICSP-081Canine hip dysplasia - Understanding the geneticbasis to apply Genomic Selection – A Master Thesisin Veterinary MedicineSilvia F. Volpi1, Maria Giuseppina Strillacci1, Aldo Vezzoni2, Anna Brandstetter2, Erika Frigo1, Maria Cristina Cozzi1, Fausta Schiavini1, Armand Sanchez3,Alessandro Bagnato11Dipartimento di Scienze Veterinarie per la Salute, la ProduzioneAnimale e la Sicurezza Alimentare, Università degli Studi diMilano, Italy2Clinica Veterinaria Vezzoni srl, Cremona, ItalyCorresponding author: alessandro.bagnato@unimi.itCanine hip dysplasia (CHD) is the most prevalent orthopaedicdisease in large breeds of dogs such as the German ShepherdDog, Golden and Labrador Retriever, Bernese Mountain Dog. It ischaracterized by an abnormal formation of the hip joints leadingdifferent degrees of laxity and subluxation with subsequent for-mation of progressive osteoarthritis. Nowadays the CHD diagno-sis is carried out by physical examination, including the evalua-tion of hip pain, and the degree of hip laxity with the Ortolanitest in young dogs, while in mature dogs the range of motion andpain of hip joint are evaluated. Moreover, the X-rays are essentialto confirm the diagnosis and to establish the severity of the dis-ease. Hip scoring (classes from A to E, according to increasingseverity in the FCI classification) is a procedure used to deter-mine the degree of hip dysplasia in dogs and it is the sum of thepoints awarded for each of the radiographic features of hipjoints. The results of several studies reported in literature high-light that selective breeding programs, based on genetic andgenomic information, are valid alternative to reduce the inci-dence of CHD in dogs. CHD is a complex trait, which expressionis influenced by genetic, nutritional, environmental and hormon-al factors. Several quantitative trait loci (QTL) on different chro-mosomes have been found to contribute to this trait expressionin various dog breeds, including Labrador retrievers. The aim ofthis Master Thesis in Veterinary Medicine is to target re-sequence the genome of a sample Labrador retrievers dogs in acase/control (dogs classified as A/D-E) study of CHD under aselective DNA pooling approach (SDP). Blood samples will be col-lected during the routine CHD diagnosis practices and genomicDNA will be extracted with a commercial kit. The chromosomeregions that will be investigated will be chosen on the bases ofthose reported in literature and associated with CHD. The result-ing sequencing data will help to find new genomic/genetic varia-tions which may be involved in the disease occurrence.P-081 bisEffect of genes related to meat quality traits onprogeny of selected bulls of Italian Limousine breedAlessandro Crovetti1, Silvia Ghezzi1, Stefano Biffani2, Antonio Bonelli1, Riccardo Bozzi11Dipartimento di Scienze delle Produzioni Agro-alimentari edell’Ambiente, Università degli Studi di Firenze, Italy 2Istituto di Biologia e Biotecnologia Agraria, ConsiglioNazionale delle Ricerche, Lodi, ItalyCorresponding author: riccardo.bozzi@unifi.itThe aim of this study was to analyze polymorphisms in candidategenes for meat quality traits in the Italian Limousine beef breed.Chemical (proximate analysis and fatty acid profile) and physical(sample cut, colour, pH, cooking loss, shear force and free water)traits from steaks of 97 steers progeny of 9 selected bulls wererecorded. DNA was extracted from the same animals andanalysed at 10 SNP mutations in 7 different genes: myostatin(MSTN), dyacilglycerol-O-acyltransferase (DGAT), calpain(CAPN), calpastatin (CAST), leptin (LEP), thyroglobulin (TG),fatty acid binding protein (FABP). Allele and genotype frequen-cies, heterozygosity, and Hardy-Weinberg equilibrium for eachSNP were estimated. Haplotype reconstruction through Phasesoftware was also performed for SNPs located on the same chro-mosome. The association among SNPs or haplotypes and therecorded traits has been performed through a linear mixedmodel incorporating the effect of the pedigree with the pedi-greemm package in R. Two SNPs at myostatin gene (F94L andQ204X) and one located in the thyroglobulin gene (C422T) werefixed whereas the other SNPs presented a MAF 0.05. Fixation ofthe first two SNPs might be either the result of the selection pro-gram developed so far or the bias due to the small sample size.No significant departures from Hardy-Weinberg equilibriumwere identified for the genotypes, except for the CAST282 locatedin the calpastatin gene where a moderate excess of heterozygousgenotypes was observed. Accounting for haplotypes did notchange the association results in any of the traits analyzed. Twoout of three SNPs of the CAPN gene (CAPN316 and CAPN530)and one at CAST gene (CAST282) were associated with meattenderness, free water content and fatty acid profile. Thus thecombined effect of favourable alleles for CAPN and CAST mark-ers resulted in improved meat quality traits providing further evi-dence that selection based on these markers might be feasible.There are also evidence that SNPs at FABP, DGAT and CASTgenes were associated with fat traits confirming the importantrole of these genes in lipid metabolism of intramuscular fat. Nosignificant association between LEP gene and meat quality traitshas been found. Due to the moderate size of the sample is notpossible to draw final conclusions but the result suggests todeepen the studies at the final aim of incorporating markersinformation in the selection criterion of Italian Limousine breed.

Effect of genes related to meat quality traits on progeny of selected bulls of Italian Limousine breed

https://flore.unifi.it/bitstream/2158/1052288/1/Pagine%20da%20ASPA2015.pdf

Effect of genes related to meat quality traits on progeny of selected bulls of Italian Limousine breed

Abstract

Similar works

Full text

Available Versions

Florence Research