Aim: The aim of this study is to detect the adequacy and quality of cervico-vaginal sampling in different age groups. Method: As a screening test, cervico-vaginal smears were obtained from 1179 women was admitted to Obstetrics and Gynecology Clinic of Bozok University Hospital between September 2012 and April 2013. Patients were grouped according to their ages. Patients under 20 years were designated as adolescents, patients between 20 and 44 years represented fertile period and patients 45 years and over were defined as menopausal patients. All the smears were evaluated by the same pathologist. Transformation Zone detection is required for sufficient and appropiate smear sampling. Kruskal Wallis H Tests were used for statistical analysis. Values under 0.05 were accepted as significant. Mann Whitney U test was performed for detecting the origin of significance. Results: Among 1179 patients, 14 of them were adolescents (1.18%), 752 of them belonged to fertile period group (63.78%) and 413 of them were menopausal patients (35.02%). In these groups smear sampling sufficiency and quality detected as; 64.20% in adolescents, 72.90% in fertile period and 59.95% in menopausal patients. Among smears with inflammation, 7 of them were adolescents (50%), 470 of them were in fertile period group (62,50%) and 158 cases were in menopause group (38,25%). Only 1 patient diagnosed as ASC-US (0,08%) and no other premalignant lesion was detected. There was a statistical difference between groups (p<0,05) and in the fertile group cervicovaginal samplings were more efficient. Conclusion: As the transformation zone presents in the easily screened outer portion, the more efficient sampling has been achieved in the fertile group compared to other groups