Ovitraps with eggs of Dasyhelea necrophila were detected at five localities in Uruguay during surveillance and control of Aedes aegypti. Relevant dates of oviposition in wild of this Ceratopogonidae species are included in addition to previous laboratory work on the species. Eggs of D. necrophila were deposited together with eggs of Culicidae and Psychodidae. These are the first records of the species from Uruguay.Fil: Martínez, María. Universidad de la República; Uruguay. Ministerio de Salud Pública. Sección Zoonosis y Vectores; UruguayFil: Canneva, Bruno. Universidad de la República; UruguayFil: Ronderos, Maria Marcela. Universidad Nacional de La Plata. Facultad de Ciencias Naturales y Museo. División Entomología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Centro de Estudios Parasitológicos y de Vectores. Universidad Nacional de La Plata. Facultad de Ciencias Naturales y Museo. Centro de Estudios Parasitológicos y de Vectores; Argentin

Canneva, Bruno

Martínez, María

Ronderos, Maria Marcela

Check List

English

Ovitraps with eggs of Dasyhelea necrophila were detected at five localities in Uruguay during surveillance and control of Aedes aegypti. Relevant dates of oviposition in wild of this Ceratopogonidae species are included in addition to previous laboratory work on the species. Eggs of D. necrophila were deposited together with eggs of Culicidae and Psychodidae. These are the first records of the species from Uruguay

Martínez,María

Canneva,Bruno

Ronderos,Maria

ARPHA Preprints

Diptera, Ceratopogonidae, Dasyhelea necrophila Spinelli and Rodriguez, 1999: detection of eggs in ovitraps, in Uruguay

Ovitraps with eggs of Dasyhelea necrophila were detected at five localities in Uruguay during surveillance andcontrol of Aedes aegypti. Relevant dates of oviposition in wild of this Ceratopogonidae species are included in additionto previous laboratory work on the species. Eggs of D. necrophila were deposited together with eggs of Culicidae andPsychodidae. These are the first records of the species from Uruguay

Martínez, M.

Canneva, B.

Ronderos, M. M.

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Journal of species lists and distribution Chec ListISSN 1809-127X (online edition)© 2010 Check List and AuthorsOpen Access | Freely available at www.checklist.org.brNotes oN GeoGraphic DistributioN239Figure 1. Map of Uruguay. Each Department in detail.Diptera, Ceratopogonidae, Dasyhelea necrophila Spinelli and Rodriguez, 1999: Detection of eggs in ovitraps, in Uruguay María Martínez 1,2*, Bruno Canneva 1 and Maria M. Ronderos 3 1  Universidad de la República, Facultad de Ciencias, Sección Entomología, Iguá 4225, 11400. Montevideo, Uruguay. 2  Ministerio de Salud Pública, Sección Zoonosis y Vectores, Leguizamón 3552, 11600. Montevideo, Uruguay. 3  Museo de La Plata, División Entomología, Paseo del Bosque s/n, 1900. La Plata, Argentina.*  Corresponding author. E-mail: mm@fcien.edu.uyAbstract: Ovitraps with eggs of Dasyhelea necrophila were detected at five localities in Uruguay during surveillance and control of Aedes aegypti. Relevant dates of oviposition in wild of this Ceratopogonidae species are included in addition to previous laboratory work on the species. Eggs of D. necrophila were deposited together with eggs of Culicidae and Psychodidae. These are the first records of the species from Uruguay.Subfamily  Dasyheleinae  includes  only  the  genus Dasyhelea  Kieffer,  species  of  which  are  known  to  be cacao pollinators in tropical zones. Adults feed on nectar and  vegetable  fluids.  Knowledge  of  their  immature stages, particularly eggs and first larval instars, is poor compared to the adults. Larvae live associated with algae in shallow water in rocks, tree hollows, and phytotelmic environments,  also  in  decomposing  vegetable  mass and  saline  environments  (Spinelli  and  Wirth  1993). Some species such as D. necrophila are found in artificial containers  and  can  resist  habitat  desiccation  for  about 48 hours (Ronderos et al. 2006) and D. pseudoincisurata Waugh and Wirth, 1976 has been collected from artificial containers,  in  Florida,  USA  (Hribar  1998;  Hribar  et  al. 2004).Dasyhelea  necrophila  was  described  by  Spinelli  and Rodriguez (1999) based on adults, pupae and fourth instar larva. Ronderos et al. (2003) redescribed and illustrated the fourth instar larva and pupa based on ultrastructural observations  and  Ronderos  et  al.  (2006)  gave  details of  laboratory  observations  on  morphology  and  biology under  different  conditions.  De  Villalobos  and  Ronderos (2003)  experimentally  infected  larvae  and  pupae  of  D. necrophila with larvae of Paragordius varius (Leidy, 1851), as a potentially paratenic host. Among  the  investigations  and  actions  for  control  of Aedes  aegypti  (Linnaeus,  1762)  which  are  carried  out nationally  by  the  Zoonosis  and  Vectors  section  of  the Epidemiology Department in the Ministry of Public Health of Uruguay, ovitraps are used to collect eggs of container-inhabiting mosquitoes. During these investigations, eggs of D. necrophila were encountered in some ovitraps.  The objective of this work is to provide information about the egg posture registry of D. necrophila, detected in ovitraps at five localities in Uruguay, complementing previous laboratory work recently done on this species.A total of 8,640 paddles from ovitraps was examined from  5  localities  of  Uruguay  (Figure  1)  during  Spring, Check List | Volume 6 | Issue 2 | 2010Summer and Fall: Flores and Montevideo (XII/2006-V/2007 and  X/2007-V/2008);  Florida  (II/2007-V/2007  and X/2007-V/2008);  Canelones  (XII/2006-V/2007  and X/2007-IV/2008); Maldonado (XII/2006-V/2007).The ovitraps consist of a black plastic bottle with a wide mouth (15 cm high, 500 ml) one third filled with water and a small wooden paddle where different species of aquatic Diptera can deposit their eggs (Hernández et al. 2002; García-da-Rosa et al. 2003). Traps were placed at or near ground level in the shade, in quiet places far from noisy areas and zones with intense vehicular traffic, and without competition from co-occurrence of other potential breeding sites. The paddles in the ovitraps were placed in 240Martínez et al. | Diptera, Ceratopogonidae, Dasyhelea necrophila Spinelli and Rodriguez, 1999Figure 2. Ovitrap with paddle.Table 1. Paddles of ovitraps examined: for localities with their coordinates, absolute number, average weekly and total of paddles with eggs of Dasyhelea necrophila. Check List | Volume 6 | Issue 2 | 2010a tilted position (Figure 2). Paddle dimensions were varied in height (23.6 cm – 14.0 cm), width (1.4 cm – 1.0 cm), and thickness (1.0 cm – 0.2 cm). Paddles were replaced by new ones weekly and were sent to the lab with their corresponding data, and were observed using stereoscopic microscopy, registering presence or absence of eggs. Some of  the  eggs  collected  were  raised  to  the  adult  stage  in the lab, for specific determination. From each paddle the number of eggs in each group, the quantity of egg masses, and their minimum and maximum height, in centimeters, were recorded. These samples were photographed with a Canon A520 digital camera and processed in Photoshop 7.0, and then extracted from the paddles with the aid of a scalpel, and preserved in 70 % ethanol. The examined material is deposited in Entomological Collection of the Facultad de Ciencias, Universidad de la República, Uruguay.The  eggs  observed  in  the  sampling  of  the  paddles from  ovitraps  belong  to  three  dipteran  families: Ceratopogonidae, Culicidae, and Psychodidae.The  Ceratopogonidae  identified  species  was  D. necrophila  representing  the  first  record  of  this  species from Uruguay. Prior to this study it was recorded only from Argentina and Mexico (Borkent and Spinelli 2007).From  8,640  paddles  examined,  eggs  of  this  species were  detected  on  26  of  them  (Table  1).  The  eggs  are C-shaped, grouped and glued with some kind of substance (Figure 3). The egg mass is glued to the substratum and in this way they are adequately incubated near water. It is possible that the sticky substance protects the eggs from natural  enemies  and  weather  changes.  The  egg  masses were irregularly shaped and were found on both sides of the paddle, (front side = 14, reverse = 17; edges = 5); 76.9 % of egg masses were deposited parallel to the water, 15.4 % were deposited in an L-shaped mass, 7.7 % in a band of 3.5 to 4 cm wide (Figure 4).Eight paddles had only one egg mass, three of them with 10 to 12 eggs each, one with 59 and 4 paddles showed a variable number of eggs, between 92 and 132. Only one paddle had a maximum of 22 groups of eggs, and the total amount of eggs counted was 1076 (Table 2).The  number  of  eggs  groups  per  paddle  varied  as follows: 30.8% of paddles had only one egg group; 23.2 % of paddles had 2 groups; 19.3 % had 3 groups; 7.7 % had 6 groups and 3.8 % had 5, 9, 10, 11 or 22 groups. The eggs were deposited at 5.4 cm minimum average high (1-12.4) and 6 cm maximum average high (1-12.4) from the lower tip. Height of oviposition above the waterline was not recorded, but the records allow us to suppose that the water level was very variable in the ovitraps in the different trap localities. These observations allow us to broaden awareness of the egg masses of D. necrophila in nature and geographical distribution of this species.DepartmentLatitude Capital of DepartmentLongitude Capital of DepartmentPaddles total for localitiesAverage weekly Nº of paddles Paddles with eggs of D. necrophilaFlores 33°32’20’’S 56°53’19’’W 964 16 12Florida 34°05’44’’S 56°12’51’’W 540 13 1Canelones 34°31’22’’S 56°16’40’’W 4141 69 4Maldonado 34°54’00’’S 54°57’00’’W 779 30 6Montevideo 34°51’29’’S 56°10’15’’W 2216 36 3Total 8640 164 26Figure 3. Egg mass of Dasyhelea necrophila.241Martínez et al. | Diptera, Ceratopogonidae, Dasyhelea necrophila Spinelli and Rodriguez, 1999Received: December 2009Revised: April 2010Accepted: April 2010Published online: May 2010Editorial responsibility: Cristiano Lopes-AndradeLiterature CitedBorkent,  A.  and  G.R.  Spinelli.  2007.  Neotropical  Ceratopogonidae (Diptera: Insecta); p. 1-198. In: J. Adis, J.R. Arias, G. Rueda-Delgado and  K.M.  Wnatzen  (eds.).  Aquatic  Biodiversity  in  Latin  America (ABLA). Vol. 4. Sofia: Pensoft Sofia-Moscow.De-Villalobos C. and M. Ronderos. 2003. Dasyhelea necrophila Spinelli and  Rodriguez  1999  (Diptera  Ceratopogonidae),  a  new  paratenic host of Paragordius varius (Leidy, 1851) (Gordiida, Nematomorpha). Acta Parasitologica 48: 218- 221. García-da-Rosa, E., R. Lairihoy, J.C. Leivas, W. González and D. Paulino. 2003. Monitoreo de Aedes aegypti mediante el uso de ovitrampas. Entomología y Vectores 10(4): 451-456.Hernández, S., M.J. Rodríguez, J. Vignolo, O. López, R. Salvatella, G. Willat, M. González, R. Rosa and J.C. Rodríguez-Nigro. 2002. Guía para la vigilancia de Aedes aegypti. Montevideo: Ministerio de Salud Pública. OPS/HCP/HCT/AA.URU.01/02.Hribar,  L.J.  1998.  Biological  and  morphological  notes  on  Dasyhelea pseudoincisurata. Entomological News 109(4): 282-284.Hribar, L.J., J.J. Vlach, D.J. DeMay, S.S. James, J.S. Fahey and E.M. Fussell. 2004. Mosquito larvae (Culicidae) and other diptera associated with containers, storm drains, and sewage treatment plants in the Florida Keys, Monroe County, Florida. Florida Entomologist 87(2): 199-203.Ronderos, M., G.R. Spinelli, H. Huerta and F. Díaz. 2003. Immature stages of  two  neotropical  species  of  Dasyhelea  Kieffer,  1911  (Diptera Ceratopogonidae).  Transactions  of  the  American  Entomological Society 129(2): 295-308.Ronderos,  M.M.,  G.R.  Spinelli,  and  F.  Díaz.  2006.  Observations  on the  immatures  of  Dasyhelea  necrophila  Spinelli  &  Rodriguez  in laboratory (Diptera Ceratopogonidae). Transactions of the American Entomological Society 132(3-4): 291-297.Spinelli, G.R. and E.A. Rodriguez. 1999. A new species of Dasyhelea from Argentina described as adult, pupae and fourth instar larvae (Diptera Ceratopogonidae). Neotrópica 45(113-114): 59-62.Spinelli, G.R. and W.W. Wirth. 1993. Los Ceratopogonidae de la Argentina (Insecta: Diptera). In: Z.A. de-Castellanos (ed.). Fauna de agua dulce de la República Argentina 38(3): 1-124.Acknowledgments: To Dra. G. Willat and persons and institutions who participated in detection and control of Aedes aegypti in Uruguay, to Dr. L. Hribar who give us bibliographic references and to Alba Bentos Pereira for suggestions.Figure 4. Egg mass of Dasyhelea necrophila in a paddle.Check List | Volume 6 | Issue 2 | 2010Nº of paddleNº of egg massNº of eggs. Nº of paddleNº of egg massNº of eggs Nº of paddleNº of egg massNº of eggsI 1 10 X 2 36 XIX 3 195II 1 12 XI 2 44 XX 5 117III 1 12 XII 2 62 XXI 6 54IV 1 59 XIII 2 64 XXII 6 146V 1 92 XIV 2 70 XXIII 9 179VI 1 110 XV 3 24 XXIV 10 109VII 1 111 XVI 3 98 XXV 11 111VIII 1 132 XVII 3 98 XXVI 22 1076IX 2 28 XVIII 3 99Table 2. Total of paddles with eggs of Dasyhelea necrophila, total of egg masses and total of eggs for each paddles. The knowledge of the breeding sites of different Diptera in the same habitat  is very important  to application  of methods  for  biological  integrative  control,  especially species  with  economical  and  sanitary  relevance  which share the same habitat for ontogenetic development.Ovitraps  are  a  good  entomological  instrument  for surveillance  and  control  of  dengue  vectors.  They  make favorable conditions for the rearing of Aedes aegypti and permit early detection of its presence, so knowledge of related  biological  groups  (with  economical  or  sanitary relevance  or  as  indicator  taxa  for  environmental monitoring) would be important for future studies.

Diptera, Ceratopogonidae, <i>Dasyhelea necrophila</i> Spinelli and Rodriguez, 1999: Detection of eggs in ovitraps, in Uruguay

ARPHA OAI-PMH Endpoint

Ronderos, Maria

ZENODO

Ovitraps with eggs of Dasyhelea necrophila were detected at five localities in Uruguay during surveillance and control of Aedes aegypti. Relevant dates of oviposition in wild of this Ceratopogonidae species are included in addition to previous laboratory work on the species. Eggs of D. necrophila were deposited together with eggs of Culicidae and Psychodidae. These are the first records of the species from Uruguay.</jats:p

María Martínez

Bruno Canneva

Maria M. Ronderos

Crossref

NEUROSURGERY ENTHUSIASTIC WOMEN SOCIETY

Maria Ronderos

Journal of species lists and distributionChec ListISSN 1809-127X (online edition)© 2010 Check List and AuthorsOpen Access | Freely available at www.checklist.org.brNotes oN GeoGraphic DistributioN239Figure 1. Map of Uruguay. Each Department in detail.Diptera, Ceratopogonidae, Dasyhelea necrophila Spinelli and Rodriguez, 1999: Detection of eggs in ovitraps, in Uruguay María Martínez 1,2*, Bruno Canneva 1 and Maria M. Ronderos 3 1 Universidad de la República, Facultad de Ciencias, Sección Entomología, Iguá 4225, 11400. Montevideo, Uruguay. 2 Ministerio de Salud Pública, Sección Zoonosis y Vectores, Leguizamón 3552, 11600. Montevideo, Uruguay. 3 Museo de La Plata, División Entomología, Paseo del Bosque s/n, 1900. La Plata, Argentina.* Corresponding author. E-mail: mm@fcien.edu.uyAbstract: Ovitraps with eggs of Dasyhelea necrophila were detected at five localities in Uruguay during surveillance and control of Aedes aegypti. Relevant dates of oviposition in wild of this Ceratopogonidae species are included in addition to previous laboratory work on the species. Eggs of D. necrophila were deposited together with eggs of Culicidae and Psychodidae. These are the first records of the species from Uruguay.Subfamily Dasyheleinae includes only the genus Dasyhelea Kieffer, species of which are known to be cacao pollinators in tropical zones. Adults feed on nectar and vegetable fluids. Knowledge of their immature stages, particularly eggs and first larval instars, is poor compared to the adults. Larvae live associated with algae in shallow water in rocks, tree hollows, and phytotelmic environments, also in decomposing vegetable mass and saline environments (Spinelli and Wirth 1993). Some species such as D. necrophila are found in artificial containers and can resist habitat desiccation for about 48 hours (Ronderos et al. 2006) and D. pseudoincisurata Waugh and Wirth, 1976 has been collected from artificial containers, in Florida, USA (Hribar 1998; Hribar et al. 2004).Dasyhelea necrophila was described by Spinelli and Rodriguez (1999) based on adults, pupae and fourth instar larva. Ronderos et al. (2003) redescribed and illustrated the fourth instar larva and pupa based on ultrastructural observations and Ronderos et al. (2006) gave details of laboratory observations on morphology and biology under different conditions. De Villalobos and Ronderos (2003) experimentally infected larvae and pupae of D. necrophila with larvae of Paragordius varius (Leidy, 1851), as a potentially paratenic host. Among the investigations and actions for control of Aedes aegypti (Linnaeus, 1762) which are carried out nationally by the Zoonosis and Vectors section of the Epidemiology Department in the Ministry of Public Health of Uruguay, ovitraps are used to collect eggs of container-inhabiting mosquitoes. During these investigations, eggs of D. necrophila were encountered in some ovitraps.  The objective of this work is to provide information about the egg posture registry of D. necrophila, detected in ovitraps at five localities in Uruguay, complementing previous laboratory work recently done on this species.A total of 8,640 paddles from ovitraps was examined from 5 localities of Uruguay (Figure 1) during Spring, Check List | Volume 6 | Issue 2 | 2010Summer and Fall: Flores and Montevideo (XII/2006-V/2007 and X/2007-V/2008); Florida (II/2007-V/2007 and X/2007-V/2008); Canelones (XII/2006-V/2007 and X/2007-IV/2008); Maldonado (XII/2006-V/2007).The ovitraps consist of a black plastic bottle with a wide mouth (15 cm high, 500 ml) one third filled with water and a small wooden paddle where different species of aquatic Diptera can deposit their eggs (Hernández et al. 2002; García-da-Rosa et al. 2003). Traps were placed at or near ground level in the shade, in quiet places far from noisy areas and zones with intense vehicular traffic, and without competition from co-occurrence of other potential breeding sites. The paddles in the ovitraps were placed in 240Martínez et al. | Diptera, Ceratopogonidae, Dasyhelea necrophila Spinelli and Rodriguez, 1999Figure 2. Ovitrap with paddle.Table 1. Paddles of ovitraps examined: for localities with their coordinates, absolute number, average weekly and total of paddles with eggs of Dasyhelea necrophila. Check List | Volume 6 | Issue 2 | 2010a tilted position (Figure 2). Paddle dimensions were varied in height (23.6 cm – 14.0 cm), width (1.4 cm – 1.0 cm), and thickness (1.0 cm – 0.2 cm). Paddles were replaced by new ones weekly and were sent to the lab with their corresponding data, and were observed using stereoscopic microscopy, registering presence or absence of eggs. Some of the eggs collected were raised to the adult stage in the lab, for specific determination. From each paddle the number of eggs in each group, the quantity of egg masses, and their minimum and maximum height, in centimeters, were recorded. These samples were photographed with a Canon A520 digital camera and processed in Photoshop 7.0, and then extracted from the paddles with the aid of a scalpel, and preserved in 70 % ethanol. The examined material is deposited in Entomological Collection of the Facultad de Ciencias, Universidad de la República, Uruguay.The eggs observed in the sampling of the paddles from ovitraps belong to three dipteran families: Ceratopogonidae, Culicidae, and Psychodidae.The Ceratopogonidae identified species was D. necrophila representing the first record of this species from Uruguay. Prior to this study it was recorded only from Argentina and Mexico (Borkent and Spinelli 2007).From 8,640 paddles examined, eggs of this species were detected on 26 of them (Table 1). The eggs are C-shaped, grouped and glued with some kind of substance (Figure 3). The egg mass is glued to the substratum and in this way they are adequately incubated near water. It is possible that the sticky substance protects the eggs from natural enemies and weather changes. The egg masses were irregularly shaped and were found on both sides of the paddle, (front side = 14, reverse = 17; edges = 5); 76.9 % of egg masses were deposited parallel to the water, 15.4 % were deposited in an L-shaped mass, 7.7 % in a band of 3.5 to 4 cm wide (Figure 4).Eight paddles had only one egg mass, three of them with 10 to 12 eggs each, one with 59 and 4 paddles showed a variable number of eggs, between 92 and 132. Only one paddle had a maximum of 22 groups of eggs, and the total amount of eggs counted was 1076 (Table 2).The number of eggs groups per paddle varied as follows: 30.8% of paddles had only one egg group; 23.2 % of paddles had 2 groups; 19.3 % had 3 groups; 7.7 % had 6 groups and 3.8 % had 5, 9, 10, 11 or 22 groups. The eggs were deposited at 5.4 cm minimum average high (1-12.4) and 6 cm maximum average high (1-12.4) from the lower tip. Height of oviposition above the waterline was not recorded, but the records allow us to suppose that the water level was very variable in the ovitraps in the different trap localities. These observations allow us to broaden awareness of the egg masses of D. necrophila in nature and geographical distribution of this species.DepartmentLatitude Capital of DepartmentLongitude Capital of DepartmentPaddles total for localitiesAverage weekly Nº of paddles Paddles with eggs of D. necrophilaFlores 33°32’20’’S 56°53’19’’W 964 16 12Florida 34°05’44’’S 56°12’51’’W 540 13 1Canelones 34°31’22’’S 56°16’40’’W 4141 69 4Maldonado 34°54’00’’S 54°57’00’’W 779 30 6Montevideo 34°51’29’’S 56°10’15’’W 2216 36 3Total 8640 164 26Figure 3. Egg mass of Dasyhelea necrophila.241Martínez et al. | Diptera, Ceratopogonidae, Dasyhelea necrophila Spinelli and Rodriguez, 1999Received: December 2009Revised: April 2010Accepted: April 2010Published online: May 2010Editorial responsibility: Cristiano Lopes-AndradeLiterature CitedBorkent, A. and G.R. Spinelli. 2007. Neotropical Ceratopogonidae (Diptera: Insecta); p. 1-198. In: J. Adis, J.R. Arias, G. Rueda-Delgado and K.M. Wnatzen (eds.). Aquatic Biodiversity in Latin America (ABLA). Vol. 4. Sofia: Pensoft Sofia-Moscow.De-Villalobos C. and M. Ronderos. 2003. Dasyhelea necrophila Spinelli and Rodriguez 1999 (Diptera Ceratopogonidae), a new paratenic host of Paragordius varius (Leidy, 1851) (Gordiida, Nematomorpha). Acta Parasitologica 48: 218- 221. García-da-Rosa, E., R. Lairihoy, J.C. Leivas, W. González and D. Paulino. 2003. Monitoreo de Aedes aegypti mediante el uso de ovitrampas. Entomología y Vectores 10(4): 451-456.Hernández, S., M.J. Rodríguez, J. Vignolo, O. López, R. Salvatella, G. Willat, M. González, R. Rosa and J.C. Rodríguez-Nigro. 2002. Guía para la vigilancia de Aedes aegypti. Montevideo: Ministerio de Salud Pública. OPS/HCP/HCT/AA.URU.01/02.Hribar, L.J. 1998. Biological and morphological notes on Dasyhelea pseudoincisurata. Entomological News 109(4): 282-284.Hribar, L.J., J.J. Vlach, D.J. DeMay, S.S. James, J.S. Fahey and E.M. Fussell. 2004. Mosquito larvae (Culicidae) and other diptera associated with containers, storm drains, and sewage treatment plants in the Florida Keys, Monroe County, Florida. Florida Entomologist 87(2): 199-203.Ronderos, M., G.R. Spinelli, H. Huerta and F. Díaz. 2003. Immature stages of two neotropical species of Dasyhelea Kieffer, 1911 (Diptera Ceratopogonidae). Transactions of the American Entomological Society 129(2): 295-308.Ronderos, M.M., G.R. Spinelli, and F. Díaz. 2006. Observations on the immatures of Dasyhelea necrophila Spinelli & Rodriguez in laboratory (Diptera Ceratopogonidae). Transactions of the American Entomological Society 132(3-4): 291-297.Spinelli, G.R. and E.A. Rodriguez. 1999. A new species of Dasyhelea from Argentina described as adult, pupae and fourth instar larvae (Diptera Ceratopogonidae). Neotrópica 45(113-114): 59-62.Spinelli, G.R. and W.W. Wirth. 1993. Los Ceratopogonidae de la Argentina (Insecta: Diptera). In: Z.A. de-Castellanos (ed.). Fauna de agua dulce de la República Argentina 38(3): 1-124.Acknowledgments: To Dra. G. Willat and persons and institutions who participated in detection and control of Aedes aegypti in Uruguay, to Dr. L. Hribar who give us bibliographic references and to Alba Bentos Pereira for suggestions.Figure 4. Egg mass of Dasyhelea necrophila in a paddle.Check List | Volume 6 | Issue 2 | 2010Nº of paddleNº of egg massNº of eggs. Nº of paddleNº of egg massNº of eggs Nº of paddleNº of egg massNº of eggsI 1 10 X 2 36 XIX 3 195II 1 12 XI 2 44 XX 5 117III 1 12 XII 2 62 XXI 6 54IV 1 59 XIII 2 64 XXII 6 146V 1 92 XIV 2 70 XXIII 9 179VI 1 110 XV 3 24 XXIV 10 109VII 1 111 XVI 3 98 XXV 11 111VIII 1 132 XVII 3 98 XXVI 22 1076IX 2 28 XVIII 3 99Table 2. Total of paddles with eggs of Dasyhelea necrophila, total of egg masses and total of eggs for each paddles. The knowledge of the breeding sites of different Diptera in the same habitat is very important to application of methods for biological integrative control, especially species with economical and sanitary relevance which share the same habitat for ontogenetic development.Ovitraps are a good entomological instrument for surveillance and control of dengue vectors. They make favorable conditions for the rearing of Aedes aegypti and permit early detection of its presence, so knowledge of related biological groups (with economical or sanitary relevance or as indicator taxa for environmental monitoring) would be important for future studies.

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Diptera, Ceratopogonidae, Dasyhelea necrophila Spinelli and Rodriguez, 1999: detection of eggs in ovitraps, in Uruguay

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