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Previous issue date: 2009Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Morfologia e Morfogênese Viral. Rio de Janeiro, RJ. Brasil.Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Morfologia e Morfogênese Viral. Rio de Janeiro, RJ. Brasil.Universidade Federal do Rio de Janeiro. Instituto de Ciências Biomédicas. Departamento de Histologia e Embriologia,. Rio de Janeiro, RJ, Brasil.Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Morfologia e Morfogênese Viral. Rio de Janeiro, RJ. Brasil.Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Morfologia e Morfogênese Viral. Rio de Janeiro, RJ. Brasil.Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Flavivírus. Rio de Janeiro, RJ. Brasil.Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Flavivírus. Rio de Janeiro, RJ. Brasil.Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Morfologia e Morfogênese Viral. Rio de Janeiro, RJ. Brasil.Histological and ultrastructural alterations in lung of BALB/c mice reinfected
with heterologous DENV by the intravenous route with non-neuroadapted dengue
viruses serotypes 1 and 2 were analyzed. The lung samples were processed following
the standard techniques of photonic and transmission electron microscopy.
Morphological studies showed breakdown of alveolocapilar barrier leading to alveolitis,
focal zones of collapse, and intraalveolar hemorrhage. Inside alveolar septa, congested
capillaries exhibited neutrophils and platelets. Alveolar capillary endothelial cells
exhibited aspects of activation with exuberant phylopodia and intracytoplasmic vesicles
and vacuoles. Morphometrical analyses demonstrated an increase of the surface density
of interalveolar septa in all reinfected mice while alveolar space density was decreased.
All these morphometrical data corroborated the histological features emphasizing the
alveolocapilar breakdrown in dengue infected animals. Important is the fact that
reinfection leads to intraalveolar fibrogenesis as demonstrated by histomorphometry and
ultrastructural studies. DENV particles, antigens and RNA were observed 72 hours
post-reinfection in mosquito cells (C6/36) inoculated with sera of the animals. The
morphological alterations observed in lungs were similar to the observed in human
cases of dengue fever and dengue hemorrhagic fever. The present study demonstrates
that the BALB/c mice, during the secondary infection by a heterologous serotype of
DENV, develop morphological alterations in lung tissue more severe than those
observed in the primary infection
