Prevalence of homozygosity for the deleted alleles of glutathione S-transferase mu (GSTM1) and theta (GSTT1) among distinct ethnic groups from Brazil: relevance to environmental carcinogenesis?

Abstract

Marilda de Souza Gonçalves “Documento produzido em parceria ou por autor vinculado à Fiocruz, mas não consta à informação no documento”.Submitted by Ana Maria Fiscina Sampaio ([email protected]) on 2017-06-06T18:07:25Z No. of bitstreams: 1 Arruda VR Prevalence of homozygosity.....pdf: 546897 bytes, checksum: 2d475ef7e94a0a8d19354493a1c6c71d (MD5)Approved for entry into archive by Ana Maria Fiscina Sampaio ([email protected]) on 2017-06-07T16:07:29Z (GMT) No. of bitstreams: 1 Arruda VR Prevalence of homozygosity.....pdf: 546897 bytes, checksum: 2d475ef7e94a0a8d19354493a1c6c71d (MD5)Made available in DSpace on 2017-06-07T16:07:29Z (GMT). No. of bitstreams: 1 Arruda VR Prevalence of homozygosity.....pdf: 546897 bytes, checksum: 2d475ef7e94a0a8d19354493a1c6c71d (MD5) Previous issue date: 1998State Universtty of Campinas. Hematology-Hernotherapy Center. Campinas, SP, BrasilState Universtty of Campinas. Hematology-Hernotherapy Center. Campinas, SP, BrasilFederal University of Bahia. Saivador, BA, BrasilEvandro Chagas Institute. Belélm, PA, BrasilEvandro Chagas Institute. Belélm, PA, BrasilState Universtty of Campinas. Hematology-Hernotherapy Center. Campinas, SP, BrasilState Universtty of Campinas. Hematology-Hernotherapy Center. Campinas, SP, BrasilEnvironmental related diseases due to occupational carcinogens and toxic substances are a serious problem particularly in developing countries. The glutathione S-transferase system is fundamental for the detoxification of numerous carcinogens and mutagens. The individual inherited susceptibility to chemical carcinogenesis due to glutathione S-transferase mu (GSTMl) and theta (GSTTl) varies significantly among distinct ethnic groups. In this study we determined the prevalence of the null genotype of the GSTMl and GSTTl genes among individuals from three distinct Brazilian racial groups using a multiplex- PCR methodology. The results showed that the highest prevalence of the null genotype for the GSTMl occurred among Caucasians (55%, allele frequency = 0.74), followed by 33% among Brazilian Black subjects (allele frequency = 0.57). and 20% among Amazonian Indians (allele frequency = 0.45). For GSTTl a homogenous distribution of the null genotype was found among Caucasian and African descendants (18.5 and 19% homozygotes, respectively, allele. frequency = 0.43). with a lower prevalence among Amazonian Indians (1 1% of homozygotes, a!lele frequency = 0.34). Whether the deficiency of the GST system contributes to a predisposition to environmental related carcinogenesis in I specific popuiations in Brazil remains to be determined