Transactions of The Royal Society of Tropical Medicine and Hygiene
Abstract
Submitted by Gilvan Almeida ([email protected]) on 2016-12-07T12:03:28Z
No. of bitstreams: 2
license.txt: 1748 bytes, checksum: 8a4605be74aa9ea9d79846c1fba20a33 (MD5)
Amoebiasis.pdf: 87301 bytes, checksum: b7ef476072b55356d681cba66adfccf2 (MD5)Rejected by Éder Freyre ([email protected]), reason: Refazer on 2017-02-14T13:21:22Z (GMT)Submitted by Gilvan Almeida ([email protected]) on 2019-02-05T17:57:23Z
No. of bitstreams: 2
Amoebiasis.pdf: 87301 bytes, checksum: b7ef476072b55356d681cba66adfccf2 (MD5)
license.txt: 1748 bytes, checksum: 8a4605be74aa9ea9d79846c1fba20a33 (MD5)Approved for entry into archive by Regiane Silva ([email protected]) on 2019-02-11T13:58:15Z (GMT) No. of bitstreams: 2
Amoebiasis.pdf: 87301 bytes, checksum: b7ef476072b55356d681cba66adfccf2 (MD5)
license.txt: 1748 bytes, checksum: 8a4605be74aa9ea9d79846c1fba20a33 (MD5)Made available in DSpace on 2019-02-11T13:58:15Z (GMT). No. of bitstreams: 2
Amoebiasis.pdf: 87301 bytes, checksum: b7ef476072b55356d681cba66adfccf2 (MD5)
license.txt: 1748 bytes, checksum: 8a4605be74aa9ea9d79846c1fba20a33 (MD5)
Previous issue date: 2004Fundação Oswaldo Cruz. Escola Nacional de Saúde Pública Sergio Arouca. Rio de Janeiro, RJ, Brasil.Fundação Oswaldo Cruz. Escola Nacional de Saúde Pública Sergio Arouca. Rio de Janeiro, RJ, Brasil.Fundação Oswaldo Cruz. Escola Nacional de Saúde Pública Sergio Arouca. Rio de Janeiro, RJ, Brasil.University of Nebraska. School of Natural Resource Sciences. Lincoln, NE, USA.Fundação Oswaldo Cruz. Escola Nacional de Saúde Pública Sergio Arouca. Rio de Janeiro, RJ, Brasil / Universite de Reims. U. F. R. de Pharmacie. Laboratoire de Paleoparasitologie. Reims, FranceUniversite de Reims. U. F. R. de Pharmacie. Laboratoire de Paleoparasitologie. Reims, France.Universite de Reims. U. F. R. de Pharmacie. Laboratoire de Paleoparasitologie. Reims, France.Fundação Oswaldo Cruz. Escola Nacional de Saúde Pública Sergio Arouca. Rio de Janeiro, RJ, Brasil.Fundação Oswaldo Cruz. Escola Nacional de Saúde Pública Sergio Arouca. Rio de Janeiro, RJ, Brasil.The identification of parasites in ancient human faeces is compromised by differential preservation of identifiable parasite structures. However, protein molecules can survive the damage of the environment and can be detected even after centuries. In this paper it is shown that is possible to detect copro-antigen of Entamoeba histolytica in historic and prehistoric human faecal remains, using a commercially available enzyme immunoassay (ELISA) kit. The kit uses monoclonal antibody-peroxidase conjugate specific for E. histolytica adhesin. A total of 90 specimens of desiccated faeces found in mummies and ancient organic sediment from South America, North America, Africa, and Europe were examined. The ELISA detected 20 positive samples, dated to about 5300 years before present to the 19th Century ad. The positive samples are from archaeological sites in Argentina, USA, France, Belgium, and Switzerland. The detection of protozoan antigen using immunoassays is a reliable tool for the studies of intestinal parasites in the past
