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Previous issue date: 2007CNPq (No. 470841/2004-4).Fundação Oswaldo Cruz. Centro de Pesquisas René Rachou. Núcleo de Estudos em Saúde Pública e Envelhecimento. Belo Horizonte, MG, Brasil / Universidade Federal de Minas Gerais. Faculdade de Medicina. Belo Horizonte, MG, Brasil.Fundação Oswaldo Cruz. Centro de Pesquisas René Rachou. Núcleo de Estudos em Saúde Pública e Envelhecimento. Belo Horizonte, MG, Brasil / Universidade Federal de Minas Gerais. Faculdade de Medicina. Belo Horizonte, MG, Brasil.CMIC Brasil Pesquisas Clínicas. Porto Alegre, RS, Brasil.CMIC Brasil Pesquisas Clínicas. Porto Alegre, RS, Brasil/Universidade Federal do Rio Grande do Sul. Hospital de Clínicas de Porto Alegre. Porto Alegre, RS, Brasil / Hospital Moinhos de Vento de Porto Alegre. Porto Alegre, RS, Brasil.Fundação Oswaldo Cruz. Centro de Pesquisas René Rachou. Núcleo de Estudos em Saúde Pública e Envelhecimento. Belo Horizonte, MG, Brasil / Universidade Federal de Minas Gerais. Faculdade de Medicina. Belo Horizonte, MG, Brasil.Apolipoprotein E (ApoE) is one of the most extensively studied genes in the context of aging, but there are few population-based studies on ApoEpolymorphism in the elderly in developing countries. The objective of the present study was to assess ApoEallele and genotype distribution in a large elderly community-based sample and its asso-ciation with age, sex and skin color. Participants included 1408 subjects (80.8% of all residents aged ≥60 years) residing in Bambuí city, MG, Brazil. The DNA samples were subjected to the polymerase chain reaction amplification, followed by the restriction fragment length polymorphism technique, with digestion by HhaI. Analysis was carried out taking into consideration the six ApoEgenotypes (ε3/ε3, ε3/ε4, ε2/ε3, ε4/ε4, ε2/ε4, and ε2/ε2), the three ApoEalleles, and the number of ApoE4 alleles for each individual. The ε3 allele pre-dominated (80.0%), followed by ε4 (13.5%) and ε2 (6.5%). All six possible genotypes were observed, the ε3/ε3 genotype being the most frequent (63.4%). This distribution was similar to that described in other western populations. Sex was not associated with number of ApoE4 alleles. Black skin color was significantly and independently associated with the presence of two ApoE4 alleles (age-sex adjusted OR = 7.38; 95%CI = 1.93-28.25), showing that the African-Brazilian elderly have a high prevalence of the ε4 allele, as observed in blacks from Africa. No association between number of ApoE4 alleles and age was found, suggesting the absence of association of ApoEgenotype with mortality in this populatio
