v.6, n.3, p.70-80, jul./set. 2009.Submitted by Marlene Santos ([email protected]) on 2013-09-16T20:26:33Z
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Previous issue date: 2009-09Pró-Reitoria de Pesquisa e Pós-Graduação da Universidade Federal de Goiás.A doença de Chagas é considerada atualmente negligenciada e sem terapêutica com eficiência completa, o que tem incentivado a pesquisa básica. Neste trabalho objetivou-se a caracterização de um isolado de Trypanosoma cruzi obtido num paciente portador da forma cardíaca e digestiva desta doença, no Hospital das Clínicas da Universidade Federal de Goiás. Inoculou-se 40 camundongos aos 15 e 60 dias de idade com 5 x 103 ou 5 x 104 tripomastigotas/animal. A parasitemia foi analisada e outros animais foram sacrificados e fixados com parafomaldeido. Fragmentos do encéfalo, coração, pulmão, língua, esôfago, estomago, rim, bexiga, intestino delgado e grosso foram incluídos em parafina, seccionados e corados com hematoxilina/eosina. Animais submetidos ao maior inóculo apresentaram ganhos de pesos menores e valores parasitêmicos mais elevados. Ocorreu parasitismo cardíaco, processo inflamatório focal ou difuso com raras alterações nos outros órgãos e ausência de mortes sugerindo a classificação deste isolado como cepa do tipo III.The Chagas disease is considered now neglectful and without therapeutics with efficiency completes what has been motivating the basic research. In this work the characterization of an isolated of Trypanosoma cruzi was aimed at obtained in a patient bearer in the heart and digestive way of this disease in the Clinics Hospital, Federal University of Goiás. It was inoculated 40 mice to the 15 and 60 days of age with 5 x 103 or 5 x 104 trypomastigotes for animal. The parasitemia was analyzed and other animals were sacrificed and fastened with formic aldehyde. Fragments of the encephalon, heart, lung, tongue, esophagus, stomach, kidney, bladder, small and thick intestine were included in paraffin, split up and red-faced with hematoxilin/eosin. Animals submitted to the largest inoculate presented won of smaller weights and values higher parasitize. It happened heart parasitism, focal lawsuit or diffuse, rare alterations in the other analyzed organs and absence of deaths suggesting the classification of this isolated as stump of the type III
