This study was conducted to determine the effect of microbial or antimicrobial additives on the performance and organ morphology of broilers raised in batteries or in floor pens. The effect of microbial additives on the presence of oocysts in the litter was also studied. Experiments 1 and 2 consisted of four treatments (non-supplemented control diet or diet supplemented with avilamycin, bacitracin methylene disalicylate or enramycin) and six repetitions in a randomized block design. In Experiment 1, 288 day-old chicks were housed in heated batteries in a environmentally controlled room, 12 chicks per cage; in Experiment 2, 1,200 day-old chicks were housed in a curtain-sided experimental house, with concrete floor and rice hulls as litter material, 50 chicks per pen. Experiments 3 and 4 were carried out similarly to Experiments 1 and 2, respectively, but the treatments consisted of microbial additives (non-supplemented control diet or Bacillus subtilis added to the feed plus Lactobacillus reuteri and Lactobacillus johnsonii added to the water, undefined microflora added to the water or live yeast added to the feed). The antibiotics did not affect the performance of birds raised in batteries, but improved feed conversion, weight gain and live weight when chickens were kept on the floor pens. Microbial additives did not affect bird performance in any environment; however, treatments affected liver weight. Microbial agents increased intestinal weight in floor-raised broilers. No relationship was seen between the use of microbial additives and the presence of oocysts in the litter