Super-resolution fluorescence microscopy offers good sensitivity and high temporal resolution for characterizing cellular structures and biomolecules. Red-emittig fluorescent dyes are ideal fluorophores for biomolecule labeling, due to reduced absorbance and autofluorescence of cells in far-red or near-red window. Here we synthesized novel fluorescent dyes based on carbopyronine core. The dyes were synthesized from two fragments based on cyclocondensation and/or Friedel-Crafts alkylation strategies
