Compression of three methods of polymerase chain reaction, culture and rapid urease test in diagnosis of Helicobactr pylori in gastric biopsy specimen

Abstract

Introduction: Helicobactr pylori (H.pylori) infection is related to chronic gastritis, peptic ulcer andduodenal ulcer. Thus, identification and treatment of the infection has a considerable importance. The aimof this study was to compare three methods of polymerase chain reaction (PCR), culture and rapid ureasetest (RUT) in identification of Helicobactr pylori in gastric biopsy specimens.Material and Methods: 263 patients (157 woman and 106 man) who suffering from digestivecomplaints and referred to the endoscopy department of Hajar Hospital in Sharkord (2007, Iran) wereparticipated in the study. Three gastric biopsy samples were collected from each patient. Samples wereexamined by standard RUT and culture methods for diagnosis of H. pylori. PCR was used for diagnosisof ureC gene.Results: Out of 263 patients, 36.49%, 9.50%, 13.30%, 9.48% and 5.50% had criteria gastritis, gastriculcer, duodenal ulcer, esophagitis and gastric cancer, respectively. H.pylori infection was diagnosed in54.37%, 31.94% and 84.79% of the patients by RUT, culture and PCR method, respectively.Conclusion: Findings of this study indicated that PCR has a greater sensitivity rather than rapid ureasetest and culture methods for diagnosis of H.pylori