Backgound : With advances in medicine and materials engineering, biomaterials, such as
silicone and titanium are used in many tissue regeneration cases. Many studies are being
conducted to confirm the reactivity of cells to various biomaterials. Among these studies,
fibroblast proliferation and differentiation are known as important factors related to capsulation.
In this study, we attempted to determine how various sized polyurethane acrylate (PUA)
nanopillar structures act on fibroblast proliferation and differentiation.
Methods : The scaffolds were prepared in four groups: flat and nanopillar (350 nm, 500 nm,
1,000 nm). Flat group was used as a control. To evaluate the effect of scaffolds of nanopillar
structures on fibroblasts, quantitatively analyzed for the degree of adhesion, proliferation and
alpha-smooth muscle actin (α-SMA) expression of cells.
Results : As a result of the MTT assay, the proliferation was lower than that of the flat group
in the nanopillar group. As a result of staining, the intensity of F-actin was significantly different
from that of the flat group. And the intensity of α-SMA was different from that of the flat group
but not significantly.
Conclusions : The nanopillar structure of PUA material seems to play a role in inhibiting
the proliferation and differentiation of fibroblasts regardless of the size of the structure. This
suggests that the use of PUA nanopillar implants may be effective in suppressing fibrosis due
to fibroblast proliferation and differentiation.ope
