The objective of our study was to verify experimentally the possibility of using coal mine drainage and gypsum from the stabilizate (the final product from the combustion desulphurisation) as the source of sulphate for the cultivation of SRB with the prospect of: purging of mine waste waters inclusive sulphates, recycling of desulphurisation agent (limestone) and production of elemental sulphur from hydrogen sulphide. The results confirmed the theoretical assumptions on the use of gypsum, which forms the substantial component of stabilizate, as the source of sulphate for sulphate-reducing bacteria, which produce hydrogen sulphide in the process of bacterial reduction of sulphates. They also showed the possibility of recycling the desulphurisation agent limestone, as well as the realistic alternative of using stabilizate in the production of elemental sulphur which still represents an important raw material needed in chemical, paper or other industries
