Introduction: Toxoplasmosis is a most common parasitic infection in humans and animals, it has two acute and chronic phases that related to IgM and IgG, respectively. This prevalence is affected by different variables, so determination of the prevalence of serum IgG and IgM antibodies against Toxoplasma in terms of these variables like age, occupation and education level is so important.Material and Methods: This descriptive-analytic cross sectional study was done on 350 pregnant women referred to health - Therapeutic centers of Hamadan city. After obtaining informed consent from volunteers, their serum samples were tested by IgG ELISA and IgM ELISA methods and their associations with age, occupation and education level variables were measured.Results: From total of 350 pregnant women, 105 cases (30%) had anti-Toxoplasma IgG, and 3 cases (2.9%) had anti-Toxoplasma IgM. Antibody titer of IgM with variable age, occupation and education level was not significant, but antibody titer of IgG with these variables was significant.Conclusion: Given the significant association between the disease and age, occupation and education level in pregnant women, it should be provide the necessary training and knowledge about preventiion and avoid of being infected with toxoplasmosis infection