We propose to use organic and mixed solvents for nuclear magnetic resonance
microscopy of fixed tissue as a means for improving image information
content. NMR properties of some standard solvents (methanol, acetone, DMSO)
and solvents in use for tissue processing in pathology (xylenes, paraffin,
‘Clearify’) have been measured, reviewed, and analyzed. It was found that
DMSO and paraffin are very useful solvents that provide images of better
quality than those obtained in water (neutralized formalin buffer). This is
illustrated on the formalin fixed mouse brain sections imaged at 16.4 teslas
(700 MHz)
