Typhoid fever causes significant biochemical changes and hepatic complications. As many studies have indicated several biochemical parameters that are involved in developing the risk of typhoid fever. The current study was designed to evaluate these risk factors in general Pakistani population. Serum biochemistry and liver enzymes were studied to investigate the relationship of these risk factors to Typhoid fever. Total 100 subjects were studied, 50 healthy individuals and 50 typhoid patients. Blood samples were collected from Allied and National Hospital, Faisalabad, Pakistan. In this study, Nested PCR was used to test the samples. Elevated level of ALT (P<0.0001) and AST (P<0.0001) were observed in typhoid patients. Typhoid patients had significantly higher concentrations of Triglyceride (P=0.0044), Globulin (P=0.0004) and Total protein (P=0.0978) while LDL (P=0.0197), Albumin (P<0.0001), Glucose (P=0.0006), HDL-cholesterol (P<0.0001) and Cholesterol (P=0.04) were significantly lower than those of healthy individuals. This study appears to be ample evidence based on the physiological and biochemical parameters in typhoid patients to explain influence of typhoid morbidity. Extensive research in this field would enable us to make modern drugs to treat typhoid fever patients
