The Investigation of Decontamination Effects of Ozone Gas on Microbial Load and Essential Oil of Several Medicinal Plants

Abstract

<span style="font-family: Times New Roman; font-size: small;"> </span><p style="margin: 0in 0in 10pt;" class="MsoNormal"><span style="color: #211d1e; line-height: 115%; font-size: 10pt;"><span style="font-family: Calibri;">Today, Ozone as a disinfectant method, without putting on the harmful effects on human and plant products, it is alternative common methods for disinfection of plant material. The research as a factorial experiment was conducted on the basis of randomized complete block design with three replications and the effects of Ozone gas on decreasing the microbial load of some important medicinal plants include: Peppermint (</span></span><span style="color: #211d1e; line-height: 115%; font-family: &quot;Garamond Premr Pro&quot;,&quot;serif&quot;; font-size: 10pt; mso-bidi-font-family: &quot;Garamond Premr Pro&quot;; mso-bidi-font-style: italic;">Mentha piperita</span><span style="color: #211d1e; line-height: 115%; font-size: 10pt;"><span style="font-family: Calibri;">), Summer savory (</span></span><span style="color: #211d1e; line-height: 115%; font-family: &quot;Garamond Premr Pro&quot;,&quot;serif&quot;; font-size: 10pt; mso-bidi-font-family: &quot;Garamond Premr Pro&quot;; mso-bidi-font-style: italic;">Satureja hortensis</span><span style="color: #211d1e; line-height: 115%; font-size: 10pt;"><span style="font-family: Calibri;">), Indian valerian(</span></span><span style="color: #211d1e; line-height: 115%; font-family: &quot;Garamond Premr Pro&quot;,&quot;serif&quot;; font-size: 10pt; mso-bidi-font-family: &quot;Garamond Premr Pro&quot;; mso-bidi-font-style: italic;">Valeriana wallichii</span><span style="color: #211d1e; line-height: 115%; font-size: 10pt;"><span style="font-family: Calibri;">), Meliss (</span></span><span style="color: #211d1e; line-height: 115%; font-family: &quot;Garamond Premr Pro&quot;,&quot;serif&quot;; font-size: 10pt; mso-bidi-font-family: &quot;Garamond Premr Pro&quot;; mso-bidi-font-style: italic;">Melissa officinalis</span><span style="color: #211d1e; line-height: 115%; font-size: 10pt;"><span style="font-family: Calibri;">) and Iranian thyme (</span></span><span style="color: #211d1e; line-height: 115%; font-family: &quot;Garamond Premr Pro&quot;,&quot;serif&quot;; font-size: 10pt; mso-bidi-font-family: &quot;Garamond Premr Pro&quot;; mso-bidi-font-style: italic;">Zataria multiflora</span><span style="font-family: Calibri;"><span style="color: #211d1e; line-height: 115%; font-size: 10pt;">) were investigated. Medicinal plants leaves were treated with Ozone gas concentration 0.3, 0.6 and 0.9 ml/L at times of 10 and 30 then total count, coliform and mold and yeast of the samples were studied. The result showed that Ozone gas decreases microbial load of medicinal plants samples. But Ozone gas and Ozone gas in medicinal plants interaction effect had no effect on essential oil content. The lowest and the highest of microbial load were detected in samples treated with concentration of 0.9 ml/L of Ozone gas and control respectively. The highest and the lowest of microbial load were observed in Iranian thyme and Indian valerian respectively. Also result showed that Ozone gas treatment for 30 min had the greatest of effect in reducing the microbial load and 0.9 ml/L Ozone gas concentration had the lowest of microbial load. Results of this survey reflect that the use of Ozone as a method of disinfection for medicinal plants is a decontamination.</span></span></p><span style="font-family: Times New Roman; font-size: small;"> </span