DNA assembly standards: Setting the low-level programming code for plant biotechnology

Abstract

[EN] Synthetic Biology is defined as the application of engineering principles to biology. It aims to increase the speed, ease and predictability with which desirable changes and novel traits can be conferred to living cells. The initial steps in this process aim to simplify the encoding of new instructions in DNA by establishing low-level programming languages for biology. Together with advances in the laboratory that allow multiple DNA molecules to be efficiently assembled together into a desired order in a single step, this approach has simplified the design and assembly of multigene constructs and has even facilitated the automated construction of synthetic chromosomes. These advances and technologies are now being applied to plants, for which there are a growing number of software and wetware tools for the design, construction and delivery of DNA molecules and for the engineering of endogenous genes. Here we review the efforts of the past decade that have established synthetic biology workflows and tools for plants and discuss the constraints and bottlenecks of this emerging field.Marta Vazquez-Vilar is funded by Wageningen University & Research. Diego Orzaez is funded by the Spanish Ministry of Economy and Competitiveness [grant number BIO2016-78601-R]. Nicola Patron is funded by the UK Biotechnological and Biological Sciences Research Council (BBSRC) and Engineering and Physics Research Council (EPSRC)Synthetic Biology for Growth programme [OpenPlant Synthetic Biology Research Centre, grant number BB/L0I4130/1], and by the Earlham DNA Foundry [grant number BB/CCG1720/1].Vázquez-Vilar, M.; Orzáez Calatayud, DV.; Patron, N. (2018). DNA assembly standards: Setting the low-level programming code for plant biotechnology. Plant Science. 273:33-41. https://doi.org/10.1016/j.plantsci.2018.02.024S334127