This study was conducted to demonstrate some hematological and blood biochemical profile of Iraqi riverine buffaloes (Bubalus bubalis) during pregnancy. Ten out of 22 adult buffaloes of 4.6 ± 0.97 years old were used in this study. Blood samples was collected from each buffalo from mating (day 0), day 22-24 PM, 10-12 days interval during gestation period. Hemoglobin (Hb) was greater (P < 0.05) at days 103 - 106, 133 - 136 and 238 - 241 of pregnancy than those at 32 -34 days PM. The AST was highest at days 133 - 136 of pregnancy. Higher and lower ALP activity was noted at days 178-181 of gestation. A considerable increase in plasma albumin occurred at days 268 - 271 of gestation in comparison to days 42 - 44 PM. In conclusion, nutritional deficiencies and metabolic disorders during gestation in riverine buffaloes can be detected by monitoring blood alterations
