The goal of this work is to study the effect of different extracting solvents on antioxidant activity, total phenolic content (TPC) and total flavonoids content (TFC) of Butternut squash skin extracts. The solvents used for extraction were acetone, methanol, and water. The TPC was estimated using Folin-Ciocalteu method and their amounts in the extracts varied from 150.09 ± 4.89 to 359.61 ± 5.77 mg GAE/ g of extract. The TFC was determined in all extracts by colorimetric AlCl3 method and their amounts varied from 0.79 ± 0.01 to 1.18 ± 0.03 mg CE/g of extract. Antioxidant activity was estimated using DPPH method. For all extracts, the antioxidant activity was expressed as percent of inhibition and IC50 values ranged from 0.21 to 1.00 mg/l.The study showed that TPC, TFC, and antioxidant activity depends on the solvent used for extraction. Interestingly, skin extracts of Butternut squash showed higher total phenolic content and higher antioxidant activities among all examined extracts. Butternut squash skin is a new precious source of phenolic compounds that can be considered as a natural antioxidant of high value for different applications
