Cloning of c-Myc gene in embryonic stem cells

Abstract

Embryonic stem cells (ESCs) are pluripotent, self-renewing cells. These cells can be used in applications such as cell therapy, drug discovery, disease modelling, and the study of cellular differentiation. In this experimental study embryonic stem cells cultured in the laboratory and were amplified. Total RNA was extracted from cells and converted to cDNA by reverse transcription-polymerase chain reaction (RT-PCR). Then c-Myc gene was amplified by (PCR) and inserted into the pTZ57R/T vector. Ligated product was transformed into susceptible bacteria and transformed bacteria were screened on a selective medium. Plasmids extracted from bacteria and enzyme digestion to confirm the sequencing was performed. The cloned c-Myc gene can be used to prepare a gene cassette to produce stem cells from somatic cell.