Hasil Peer Review Artikel Berjudul "Studies of Bacillus subtilis NAP1 to degrade BOD, COD, TSS, and pH: The Iindigenous bacteria in Indonesia batik wastewater"
Batik is an industry that is quite popular in Indonesia. Batik has been declared as one
of Indonesia's cultural heritage and is recognized by UNESCO. Batik production is recorded to
always increase every year. Unfortunately, the batik industry in Indonesia is dominated by
many small industries with limited fund management. This makes the environmental aspects
and waste disposal neglected. Through this research, study and isolation were carried out to
explore the potential of indigenous bacterial isolates that can biodegrade dyes in batik along
with other physical parameters of waste. A sample that contained wastewater and sediment
from batik industry are collected and cultured in 1000 ml Busnall Hass medium which olive
oil-enriched and put at the shaker condition at 150 rpm until 6 days at 30°C. Identification of
the isolates examined for their morphological, physiological and biochemistry test. Bacillus
subtilis is one of the indigenous isolates from this research. This study aimed to determine the
ability of B. subtilis to reduce BOD, COD, TSS, and pH in batik waste and present the results
of the DNA analysis of B. subtilis. Based on the research that has been done, it can be
concluded that the molecular identification of bacteria through analysis of the 16S rDNA gene
fragment sequence, the bacteria that play a role in the degradation of dyes is Bacillus subtilis
strain NAP1 with an average similarity level of 93%. B. subtilis NAP1. B. subtilis NAP1
isolate offers the potential for future bioremediation of batik wastewate