Initial micropropagation strawberryFragaria annaninaL. from seed

Abstract

In this study, we propagate shoots ofFragaria ananassa L.in vitrofrom seed by some kinds of cytokinin including BA (0.2-1.2 mg/L) or 2-ip (0.2-1.2 mg/L) or kinetin (0.2-1.2 mg/L). After 4 weeks, shoots are changed into root medium as MS ½ with IAA 0.5 mg/L and activated carbon 2.0 g/L in vitroor Kelp fertilizer (0-1.5 %) ex vitro. The maximum number of shoots is obtained in the basal MS supplemented with BA 0.6 mg/L. In in vitroculture, formation of roots are grown on MS ½ with IAA 0.5 mg/L, activated carbon 2.0 g/L. In ex vitroculture, formation of roots are the best on Kelp fertilizer 0,5%