Human innate immune cytokine response to dengue virus

Abstract

Fluorescence minus four controls for flow cytometry experiments of innate immune cytokine production in response to dengue virus. Pseudo color flow cytometry plots for a representative patient comparing the fluorescence minus four (FMF; top row) control with completely stained sample (bottom) for secretion of (A) TNF-α, (B) IP-10, (C) IL-10, (D) IL-6 and (E) IFN-γ from the indicated cell subsets. Abbreviations: CM – CD14+CD16- classical monocytes; IM – CD14+CD16+ intermediate monocytes; NCM – CD14-CD16+ non-classical monocytes; G – Granulocytes; NKT – CD56+CD3+ natural killer T cells; NK++ - CD56+CD16+ natural killer cells; NK+- - CD56+CD16- natural killer cells; B – CD19+ cells