“Ion Ionescu de la Brad” University of Agricultural Sciences and Veterinary Medicine, Iași
Abstract
Pollen quality is an important indicator in the estimation of grape production, being analysed based on its germination capacity. Thus, viability and germination potential of pollen from six Vitisvinifera L. varieties, grownin the climatic area of the Iasi vineyard,were analysed. To perform the determinations, were used comparatively three methods for observingthe viable cells, by treating them with tetrazolium chloride (TTC), Lugol solution (IKI) and methylene blue (AM) solution. The germinating potential was analysed in vitro, the culture being performed on agar medium with added sucrose (0 to 20%). The viable cells weremore clearly highlighted using the AM method, but the TTC method was more accurate in indicating the percentage of pollen viability. The highest pollen germination rate was observed in the 15% added sucrose variant
