The association between theca cells (TCs) and granulosa cells is pivotal to steroid biosynthesis in the ovary, but most data on canonical mechanisms of follicle steroidogenesis come from animal models. Indeed, the specificity of human steroidogenesis has not yet been documented in the ovary. Elucidating the cascade of events in human ovarian tissue would advance TC differentiation research, facilitating development of a transplantable engineered ovary