ARBONA, Jean-Michel

ARNEODO, Alain

AUDIT, B.

GOLDAR, Arach

HYRIEN, Olivier

Oskar Bordeaux

Universal temporal rate of DNA replication origin firing: A balance between origin activation and passivation

Arbona, Jean-Michel

Goldar, Arach

Hyrien, Olivier

Arneodo, Alain

Audit, B.

HAL-CEA

HAL-ENS-LYON

AbstractThe time-dependent rate I(t) of origin firing per length of unreplicated DNA presents a universal bell shape in eukaryotes that has been interpreted as the result of a complex time-evolving interaction between origins and limiting firing factors. Here we show that a normal diffusion of replication fork components towards localized potential replication origins (p-oris) can more simply account for the I(t) universal bell shape, as a consequence of a competition between the origin firing time and the time needed to replicate DNA separating two neighboring p-oris. We predict the I(t) maximal value to be the product of the replication fork speed with the squared p-ori density. We show that this relation is robustly observed in simulations and in experimental data for several eukaryotes. Our work underlines that fork-component recycling and potential origins localization are sufficient spatial ingredients to explain the universality of DNA replication kinetics.</jats:p

Universal temporal rate of DNA replication origin firing: A balance between origin activation and passivation

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Oskar Bordeaux

HAL-CEA

HAL-ENS-LYON

Oskar Bordeaux

Oskar Bordeaux

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Oskar Bordeaux