Even though calcium (Ca), strontium (Sr) and barium (Ba) belong to the same group of the periodic table of elements, and thus have similar chemical features, their importance for plants differs greatly. Since plants do not have the ability to completely discriminate between essential (e.g. Ca) and non-essential elements (e.g. Sr and Ba), they readily take all of them up from soil solution, which is reflected in the ratios of concentrations of those elements in plant tissues, and it influences their nutritive characteristics. The ability of plant species and genotypes to take up and accumulate chemical elements in their different tissues is related to their genetic background. However, differences in chemical composition are the least reflected in their reproductive parts. Hence, the aim of this study was to evaluate ratios of concentrations of Ca, Sr and Ba in the whole grain of diploid and tetraploid wheat ‒ ancestors of common wheat, as well as in hexaploid commercial cultivars, grown in the field, at the same location, over a period of three years. The investigated genotypes accumulated Ca, Sr and Ba at different levels, which is reflected in the ratio of their concentrations in the grain. The lowest ratio was established between Ba and Sr, followed by Ca and Ba, while the highest ratio was between Ca and Sr. Moreover, the results have shown that the year of study, genotype and the combination highly significantly affected the ratio of the concentration Ca:Sr, Ca:Ba, and Ba:Sr.Kalcijum (Ca), stroncijum (Sr) i barijum (Ba) pripadaju istoj grupi elemenata Periodnog sistema i imaju slične hemijske osobine. Međutim, njihove uloge u biljnom organizmu veoma se razlikuju. Obzirom da biljke nemaju sposobnost da u potpunosti razlikuju esencijalne (C a) od neesencijalnih elemenata (Sr i B a), one ih sve usvajaju iz zemljišnog rastvora, a to se odražava u različitom odnosu koncentracija ovih elemenata u biljnom tkivu i utiče na njihove nutritivne osobine. Sposobnost vrsta i genotipova da usvajaju i akumuliraju hemijske elemente u različitim tkivima, zavisi od njihove genetike. I pored toga, razlike u hemijskom sastavu ogledaju se u njihovoj reproduktivnoj ulozi. Cilj istraživanja bio je da se odrede odnosi koncentracija Ca, Sr i Ba u celom zrnu diploidne i tetraploidne pšenice - pretka današnje pšenice, kao i heksaploidnih komercijalnih vrsta, gajenih na istom polju i lokalitetu tokom tri godine. Ispitivani genotipovi su akumulirali Ca, Sr i Ba u različitim količinama, što se odrazilo na odnos njihovih koncentracija u zrnu. Najniži odnos ustanovljen je između Ba i Sr, zatim Ca i Ba, a najveći između Ca i Sr. Takođe, rezultati su pokazali da godina ispitivanja , genotip i kombinacija značajno utiču na odnos koncentracije Ca:Sr, Ca:Ba i B a:Sr