Camelina [Camelina sativa (L.) Crantz] also known as “false flax” or “gold of pleasure”, is a self-pollinated, annual oilseed that belongs to the Brassicaceae family. Camelina is native species of Eurasia, which is gaining interest world-wide due to its better cold, heat and drought tolerance, and less susceptibility to disease and pests than oilseed rape. The most of research work on camelina has been carried out in northern America and continental Europe. Consequently, there are not many data on evaluation of suitability of camelina genotypes for cultivation in southern Europe. Two breeding groups (IFVCNS and BOKU) and one group focusing on the agronomy development of the crop (DISTAL) just recently started research activities focusing on development of new genotypes more adapted for southern regions of Europe and evaluation of their productivity in these, more arid regions.The poster for this abstract is available here [http://fiver.ifvcns.rs/handle/123456789/2259

Cvejić, Sandra

Jocković, Milan

Kiprovski, Biljana

Kondić-Špika, Ankica

Kuzmanović, Boris

Marjanović-Jeromela, Ana

Miladinović, Dragana

Mladenov, Velimir

Nagl, Nevena

Rajković, Dragana

Vollmann, Johann

Zanetti, Federica

Čanak, Petar

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New technologies in achieving heat and drought resilient oilseed production, the case of camelina

Developing wheat able to sustainably produce high yields when grown under biotic/abiotic stresses is an important goal, in order to obtain food security in the face of ever-increasing human population and unpredictable global climatic conditions. However, random mutagenesis or genetic recombination as conventional ways for wheat improvement, are time-consuming and cannot keep pace with increasing food demands. Targeted genome editing (GE) technologies, like zinc-finger nucleases, transcription activator-like effector nuclease, and clustered regularly interspaced short palindromic repeats (CRISPR)/(CRISPR)-associated protein 9 (Cas9)) have been successfully used in editing wheat genome to get heritable variations for creating diversity and precision breeding. The tetraploid durum wheat (Triticum turgidum ssp. durum L.) and the hexaploid bread wheat (Triticum aestivum L.) are the most widely cultivated types, both with large genomes, developed as a consequence of ancient hybridization events between ancestral progenitors.The poster for this abstract is available here [http://fiver.ifvcns.rs/handle/123456789/2260

Mikić, Sanja

Mirosavljević, Milan

Takač, Verica

Genome editing of wheat - challenges and prospects for tackling changing environment

Developing wheat able to sustainably produce high yields when grown under biotic/abiotic stresses is an important goal, in order to obtain food security in the face of ever-increasing human population and unpredictable global climatic conditions. However, random mutagenesis or genetic recombination as conventional ways for wheat improvement, are time-consuming and cannot keep pace with increasing food demands. Targeted genome editing (GE) technologies, like zinc-finger nucleases, transcription activator-like effector nuclease, and clustered regularly interspaced short palindromic repeats (CRISPR)/(CRISPR)-associated protein 9 (Cas9)) have been successfully used in editing wheat genome to get heritable variations for creating diversity and precision breeding. The tetraploid durum wheat (Triticum turgidum ssp. durum L.) and the hexaploid bread wheat (Triticum aestivum L.) are the most widely cultivated types, both with large genomes, developed as a consequence of ancient hybridization events between ancestral progenitors.This is a poster presented at the 2nd PlantEd Conference "Plant genome editing: the wide range of applications", held on 20-22 September 2021 in Lecce, Italy. The abstract from this conference is available here: [http://fiver.ifvcns.rs/handle/123456789/2253

Ankica Kondić-Špika1, Sanja Mikić1, Milan Mirosavljević1, Verica Takač1,Dragana Miladinović1, Ana Marjanović Jeromela1Institute of Field and Vegetable Crops, Maksima Gorkog 30, 21000 Novi Sad, SerbiaGENOME EDITING OF WHEAT - CHALLENGES AND PROSPECTS FOR TACKLING CHANGING ENVIRONMENT.Developing wheat able to sustainably produce high yields when grown under biotic/abiotic stresses is an important goal, in order to obtain food security in theface of ever-increasing human population and unpredictable global climatic conditions. However, random mutagenesis or genetic recombination as conventionalways for wheat improvement, are time-consuming and cannot keep pace with increasing food demands. Targeted genome editing (GE) technologies, like zinc-finger nucleases, transcription activator-like effector nuclease, and clustered regularly interspaced short palindromic repeats (CRISPR)/(CRISPR)-associated protein9 (Cas9)) have been successfully used in editing wheat genome to get heritable variations for creating diversity and precision breeding. The tetraploid durumwheat (Triticum turgidum ssp. durum L.) and the hexaploid bread wheat (Triticum aestivum L.) are the most widely cultivated types, both with large genomes,developed as a consequence of ancient hybridization events between ancestral progenitors. The highly conserved gene sequence and structure of homoeologsamong subgenomes in wheat often permits their simultaneous targeting using CRISPR-Cas9 with single or paired single guide RNA (sgRNA). Since its firstsuccessful deployment in wheat, CRISPR-Cas9 technology has been applied to a wide array of gene targets of agronomical and scientific importance, such as α-gliadin genes to lower gluten grain content, TaGW2 to increase grain weight, TaZIP4-B2 to understand meiotic homologous crossover, TaQsd1 to reduce preharvestsprouting, TaMTL and CENH3 for haploid plant induction etc. In the future, genes important for abiotic stress tolerance of wheat should be also targeted by GEtechnologies. During the last decade, identification of sources for abiotic stress tolerance in the IFVCNS wheat collection was performed under different projects,complemented with molecular analyses for identification of candidate genes of importance for wide adaptation of wheat to changeable environments. The finalaim is the exploitation of IFVCNS wheat collections and the newest breeding technologies, such as genome editing, epigenetic tools, genome selection etc. forcreation of highly productive resilient wheat varieties, as well as ideotypes specific for certain agro-ecological conditions.Acknowledgements: This work is part of the project supported by the Ministry of Education, Science and Technological Development of Republic of Serbia, grant number 451-03-9/2021-14/200032, and COST Action CA18111.Probable traits for CRISPR-based genome editing in wheat

Camelina [Camelina sativa (L.) Crantz] also known as “false flax” or “gold of pleasure”, is a self-pollinated, annual oilseed that belongs to the Brassicaceae family. Camelina is native species of Eurasia, which is gaining interest world-wide due to its better cold, heat and drought tolerance, and less susceptibility to disease and pests than oilseed rape. The most of research work on camelina has been carried out in northern America and continental Europe. Consequently, there are not many data on evaluation of suitability of camelina genotypes for cultivation in southern Europe. Two breeding groups (IFVCNS and BOKU) and one group focusing on the agronomy development of the crop (DISTAL) just recently started research activities focusing on development of new genotypes more adapted for southern regions of Europe and evaluation of their productivity in these, more arid regions.This is a poster presented at the 2nd PlantEd Conference "Plant genome editing: the wide range of applications", held on 20-22 September 2021 in Lecce, Italy.
The abstract from this conference is available here: [http://fiver.ifvcns.rs/handle/123456789/2252

Ana Marjanović Jeromela1, Ankica Kondić Špika1, Dragana Rajković1, Sandra Cvejić1, Milan Jocković1, Federica Zanetti2, Johann Vollmann3, Nevena Nagl1, Bijana Kiprovski1, Petar Čanak1, Boris Kuzmanoviċ4, Velimir Mladenov4, Dragana Miladinović11Institute of Field and Vegetable Crops (IFVCNS), Novi Sad, Serbia2Dept. of Agricultural and Food Sciences (DISTAL), University of Bologna, Italy3University of Natural Resources and Life Sciences (BOKU), Vienna, Austria4Faculty of Agriculture, University of Novi Sad, SerbiaNEW TECHNOLOGIES IN ACHIEVING HEAT AND DROUGHT RESILIENT OILSEED PRODUCTION, THE CASE OF CAMELINA.Camelina [Camelina sativa (L.) Crantz] also known as “false flax” or “gold of pleasure”, is a self-pollinated, annual oilseed that belongsto the Brassicaceae family. Camelina is native species of Eurasia, which is gaining interest world-wide due to its better cold, heat and droughttolerance, and less susceptibility to disease and pests than oilseed rape. The most of research work on camelina has been carried out innorthern America and continental Europe. Consequently, there are not many data on evaluation of suitability of camelina genotypes forcultivation in southern Europe. Two breeding groups (IFVCNS and BOKU) and one group focusing on the agronomy development of the crop(DISTAL) just recently started research activities focusing on development of new genotypes more adapted for southern regions of Europe andevaluation of their productivity in these, more arid regions.The hexaploid oilseed crop Camelina sativa, which has three closely related expressed subgenomes, is an ideal species forinvestigation of gene dosage as an important cause of phenotype variation. Targeted mutagenesis of the three delta-12-desaturase (FAD2) geneswas recently achieved in camelina by CRISPR-Cas9 gene editing, leading to combinatorial association of different alleles for the three FAD2 loci.As a result, a large diversity of camelina lines was obtained with various lipid profiles, ranging from 10% to 62% oleic acid accumulation in the oil.Using the same approach, the different allelic combinations of genes associated with heat or drought stress tolerance may provide a uniquesource of genetic variability for creation of climate resilient camelina. ‘Omics’ studies which are in progress will identify the genes of interests,proteins, and metabolites in developing seeds that are impacted by heat or drought stress. Such studies, along with effective agronomicmanagement system would pave the way in developing crop genotypes/varieties with improved productivity under drought and/or heatstresses. This would lead to prevention of high risk scenarios in the future production of oilseed crops, due to inability of the staple oilseed cropsto adapt to high temperatures and drought.Acknowledgements: This work is part of the project supported by Ministry of Education, Science and Technological Development of Republic of Serbia, grant number 451-03-9/2021-14/200032, and COST Action CA18111.Contact: Ana Marjanović Jeromela, Institute of Field and Vegetable Crops, Maksima Gorkog 30, 21000 Novi Sad, Serbia. Email: ana.jeromela@ifvcns.ns.ac.rs 

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New technologies in achieving heat and drought resilient oilseed production, the case of camelina

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