Target strength (TS) of marine fish is a key factor for target identification and stock quantification. Validation of measurement and model comparisons in fisheries acoustics is difficult, due to the uncertainty in ground truth obtained in the ocean. To overcome this problem is to utilize laboratory measurements, where fish parameter is more well controlled. In this research, the dorsal‐aspect TS of fish was measured as a function of the incidence angle in a water tank using a quantitative echo sounder. The measurement was compared with the theoretical prediction using the distorted‐wave born approximation (DWBA) model. TS of fish was proportional to body length and the directivity of TS was strongly dependent on its orientation. Computational DWBA modeling, experimental details, and data/model comparison were presented