Two bionanocomposites based on suture threads, silk-silver nanoparticles (Ag NPs) and catgut-Ag NPs, were prepared through a green chemistry methodology using Chenopodium ambrosioides (Mexican Epazote) as reducing agent. UV-Vis spectrophotometry (UV-Vis), Scanning Electron Microscopy (SEM) and Transmission Electron Microscopy (TEM), were used for their characterization. UV-Vis confirmed the synthesis of silver nanoparticles. Micrographs showed polydisperse, mostly spherical, Ag NPs attached to both suture threads. The bionanocomposites antimicrobial properties were evaluated through cultures and inhibition zones tests. The Chenopodium ambrosioides-assisted synthesized bionanocomposites have proved antibacterial effect against S. aureus and E. coli in both sutures (silk and catgut) and could be potentially useful for oral or periodontal surgery. There was no significant difference statistically in inhibition of Staphylococcus aureus versus Escherichia coli
