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专一性脱硫菌脱硫活性比较与基因保守性研究
Authors
刘会洲
李信
+3 more
李望良
熊小超
邢建民
Publication date
1 January 2005
Publisher
Abstract
对几株能专一性脱除二苯并噻吩(DBT)中硫元素生成2-羟基联苯的细菌,即短芽孢杆菌(Bacillus brevis)R-6、德氏假单孢菌(Pseudomonas delafleldii)R-8、小球诺卡氏菌(Nocardia globerula)R-9、球形芽孢杆菌(Bacillus sphaericus)R-16、红平红球菌(Rhodococcus erythropolis)LSSE8-1和戈登氏菌(Gordonia nitida)LSSEJ-1展开研究.对照研究发现它们对DBT及其衍生物的代谢活性存在着一定的差异.为了从基因水平分析造成这些差别的原因,对这几株菌的脱硫基因展开了研究.根据Rhodococcus erythropolis IGTS8脱硫基因的保守区设计引物,PCR扩增了R-6、R-8的脱硫基因.测序结果表明脱硫基因高度保守,与IGTS8的相关脱硫基因相似性在99%以上.为了进一步验证不同专一性脱硫菌的脱硫基因的保守性,PCR扩增、克隆了LSSEJ-1和R-9的整个脱硫操纵子,结果表明脱硫基因在这两株菌中也是高度保守的.与IGTS8的相关脱硫基因相比较:R-9的dszA与IGTS8的dszA同源性为99.6%,LSSEJ-1的dszA与IGTS8的dszA的同源性为99.9%;R-9和LSSEJ-1的dszB的同源性与IGTS8的dszB都是99.9%;R-9的dszC与IGTS8的dszC同源性是99.9%,LSSEJ-1的dszC与IGTS8的dszC同源性为99.1%.对比研究认为专一性脱硫嗜温菌的脱硫基因的起源可能相同
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Last time updated on 11/04/2021