The study was conducted with the objective to identify adaptable high biomass, leaf to steam ratio and good quality yield of Alfalfa cultivars at Bore Agricultural research center. Eight Alfalfa cultivars; Hunter river, Magna-801-FG, Pioneer (1995) DZF-406, Segule1396 (408), Peruvian DZF (406), F-G-9-09, F-L-L-77-(406) and Hunter river (4010) were tested in RCBD with three replications. Plant height was significantly (P < 0.05) higher for Hairy (1995) DZF-406 cultivar (83 cm) while lower for Magna-801-FG. Disease resistance was significantly differ (P < 0.05) among cultivars. Segule (1396)-408 cultivars were highly resistant to diseases compared to others. Leaf to stem ratio, biomass yield and seed yield were not show significant (P > 0.05) different among treatments. Regarding chemical compositions of NDF, ADL and CP were significantly (P < 0.05) different among treatments. The superior ADL was obtained from Segule (1396)-408 cultivar (32.5%) while, low was obtained from F-L-L-77 (406) cultivar. The superior NDF was obtained from Poineer (1995) DZF-406 cultivar (70.7%) whereas; the low was from Peruvien- DZF- 406 cultivar (45.9%). The CP yield showed significant (P < 0.05) difference among cultivars. Magna- 801-FG cultivar had the superior (26.3%) CP while, Segule (1396)-408 cultivar (11.3%) had the inferior CP content. Cultivar F-G-9-09 was produce superior in DM (91.8%) and less in ADL (9.3%) while Segule 1396 (1396)-408 cultivar produce (91.3%) DM and lower in TASH (10.8%). superior OM was obtained from Segule 1396 (408) cultivar (80.4%) compared to others. Based on its yield, plant height, biomass yield and chemical compositions, good DM and OM Peruvien- DZF- 406, F-L-L-77 (406,) Segule-1396 (408) and F-G-9-09 is recommended for further promotion in the highland and midland of Guji and similar agro-ecologies. Keywords: Medicago Sativa, chemical composion, Adola, Cultivar. DOI: 10.7176/FSQM/106-03 Publication date:March 31st 202