Following publication of the original article [1], it has been brought to the authors' attention that in their paper (Rodrigues et al. 2016) they reported the genome size based on 2C values (diploid genome) when it is more common to present it as 1C value

Borges, Paulo AV

Castro, Mariana

Dietrich, Christopher H

Gharbi, Karim

Jiggins, Chris

Johnson, Kevin P

Loureiro, João

Paulo, Octávio S

Pina-Martins, Francisco

Quartau, José A

Rodrigues, Ana SB

Seabra, Sofia G

Silva, Sara E

Jiggins, Chris D

Apollo (Cambridge)

CORRECTION Open AccessCorrection to: Assessing genotype-phenotype associations in three dorsalcolour morphs in the meadow spittlebugPhilaenus spumarius (L.) (Hemiptera:Aphrophoridae) using genomic andtranscriptomic resourcesAna S. B. Rodrigues1*, Sara E. Silva1, Francisco Pina-Martins1,2, João Loureiro3, Mariana Castro3, Karim Gharbi4,Kevin P. Johnson5, Christopher H. Dietrich5, Paulo A. V. Borges6, José A. Quartau1, Chris D. Jiggins7,Octávio S. Paulo1† and Sofia G. Seabra1†Correction to: BMC Genet (2016) 17:144https://doi.org/10.1186/s12863-016-0455-5Following publication of the original article [1], it hasbeen brought to the authors’ attention that in theirpaper (Rodrigues et al. 2016) they reported the genomesize based on 2C values (diploid genome) when it ismore common to present it as 1C value.This has led to a misinterpretation of the percentageof the genome that was sequenced. However, none ofthe remaining analyses were affected.Please find below the corrections to the text (organizedper article PDF page number). The authors have alsoadded a column with the 1C values in Additional file 2:Table S5.Page 1, Abstract. Where it reads: “A partial genomeassembly, representing 24% of the total size...”, it shouldread: “A partial genome assembly, representing 48% ofthe total size...”Page 5 - Where it reads: “The homoploid genome size(2C in pg; [71]) was assessed through the formula”, itshould read: “The holoploid genome size (2C in pg; [71])was assessed through the formula”.Page 5 - Where it reads: “The obtained values wereexpressed in picograms (pg) and in giga base pairs (Gb),using the formula by [72] (1 pg = 0.978 Gb)”, it shouldread: “The obtained values were expressed in picograms(pg) and in giga base pairs (Gb), using the formula by[72] (1 pg = 0.978 Gb). The monoploid genome size(1Cx) was obtained by dividing the holoploid genomesize by the ploidy level, in this case, diploid, and was alsoexpressed in picograms and giga base pairs.”Page 9 - Where it reads: “Philaenus spumarius and P.maghresignus estimates of genome size were 5.27 ± 0.25 pg(5.15 Gb) and 8.90 ± 0.20 pg (8.90 Gb), respectively. In P.spumarius, males and females differed significantly in gen-ome size (F1,11 = 14.292, p-value = 0.0030), with malespresenting on average a lower genome size (5.07 ± 0.20 pg;4.96 Gb) than females (5.44 ± 0.15 pg; 5.33 Gb) (Additional© The Author(s). 2020 Open Access This article is licensed under a Creative Commons Attribution 4.0 International License,which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you giveappropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate ifchanges were made. The images or other third party material in this article are included in the article's Creative Commonslicence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commonslicence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtainpermission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to thedata made available in this article, unless otherwise stated in a credit line to the data.The original article can be found online at https://doi.org/10.1186/s12863-016-0455-5* Correspondence: ana87bartolomeu@gmail.com†Octávio S. Paulo and Sofia G. Seabra contributed equally to this work.1Computational Biology and Population Genomics Group, cE3c – Centre forEcology, Evolution and Environmental Changes, Departamento de BiologiaAnimal, Faculdade de Ciências da Universidade de Lisboa, Campo Grande,P-1749-016 Lisbon, PortugalFull list of author information is available at the end of the articleRodrigues et al. BMC Genetics           (2020) 21:36 https://doi.org/10.1186/s12863-020-00842-6file 2: Table S5).”, it should read: “Philaenus spumarius andP. maghresignus estimates of monoploid genome size were2.63 ± 0.13 pg (2.58 Gb) and 4.45 ± 0.10 pg (4.35 Gb), re-spectively. In P. spumarius, males and females differed sig-nificantly in monoploid genome size (F1,11 = 14.292, p-value = 0.0030), with males presenting on average a lowergenome size (2.53 ± 0.10 pg; 2.47 Gb) than females (2.72 ±0.08 pg; 2.66 Gb) (Additional file 2: Table S5).”Page 10 - Where it reads: “In total, 1,218,749,078 bpwere assembled which based on the total estimated gen-ome size of 5.3 Gb, correspond to approximately 24% ofthe P. spumarius genome.”, it should read: “In total, 1,218,749,078 bp were assembled which based on the total esti-mated monoploid genome size of 2.58 Gb, correspond toapproximately 48% of the P. spumarius genome.”Page 12 - Where it reads: “... an insect species with avery large genome (5.3 Gb)...”, it should read: “... an in-sect species with a very large genome (2.58 Gb)...”Moreover, the authors would like to inform thereaders that the raw reads of the RAD libraries used forassociation analyses are available in NCBI under the ac-cession PRJNA572593.The authors apologize to the editor and readers forany inconvenience caused by this error.Furthermore, they would like to thank Roberto Biello(John Innes Centre) and Saskia Hogenhout (John InnesCentre) for having brought this matter to their attention.Supplementary informationSupplementary information accompanies this paper at https://doi.org/10.1186/s12863-020-00842-6.Additional file 2: Table S1-S13. Lists of colour-associated SNPsobtained for each pairwise comparison and association analyses; genicand genotypic differentiation tests; pairwise Fst estimates among dorsalcolour phenotypes; SNP correlation value (r2) in linkage disequilibriumanalyses; Genome size estimates; Assembly statistics for genome andtranscriptome; and lists of blast results.Author details1Computational Biology and Population Genomics Group, cE3c – Centre forEcology, Evolution and Environmental Changes, Departamento de BiologiaAnimal, Faculdade de Ciências da Universidade de Lisboa, Campo Grande,P-1749-016 Lisbon, Portugal. 2Centro de Estudos do Ambiente e do Mar(CESAM), DBA/FCUL, Lisbon, Portugal. 3Department of Life Sciences, Centrefor Functional Ecology, University of Coimbra, Coimbra, Portugal. 4EdinburghGenomics, Ashworth Laboratories, King’s Buildings, The University ofEdinburgh, Edinburgh EH9 3JT, UK. 5Illinois Natural History Survey, PrairieResearch Institute, University of Illinois, Champaign, IL, USA. 6Departamentode Ciências e Engenharia do Ambiente, Angra do Heroísmo, cE3c – Centrefor Ecology, Evolution and Environmental Changes/Azorean BiodiversityGroup and Universidade dos Açores, Açores, Portugal. 7Department ofZoology, University of Cambridge, Downing Street, Cambridge CB2 3EJ, UK.Reference1. Rodrigues AS, et al. Assessing genotype-phenotype associations in threedorsal colour morphs in the meadow spittlebug Philaenus spumarius (L.)(Hemiptera: Aphrophoridae) using genomic and transcriptomic resources.BMC Genet. 2016;17:144. https://doi.org/10.1186/s12863-016-0455-5.Rodrigues et al. BMC Genetics           (2020) 21:36 Page 2 of 2

Correction to: Assessing genotype-phenotype associations in three dorsal colour morphs in the meadow spittlebug Philaenus spumarius (L.) (Hemiptera: Aphrophoridae) using genomic and transcriptomic resources.

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Correction to: Assessing genotype-phenotype associations in three dorsal colour morphs in the meadow spittlebug Philaenus spumarius (L.) (Hemiptera: Aphrophoridae) using genomic and transcriptomic resources.

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