Applied ergonomics is very important in minimally invasive surgery (MIS), especially with the introduction of robotized techniques that have changed the surgeons’ work conditions. However, the main aim remains the engineering to enable the compatibility of fulfillment of surgeons’ tasks in a physical, logical, and organizational environment with security, comfort, and efficiency. Ergonomics contribution is oriented both to design and redesign utilized material and to work organization. Epidemiological studies have shown the appearance of musculoskeletal pathologies in surgeons performing MIS; therefore, it is relevant to identify the intensity, frequency, and duration of risk factors (posture, repeatability, level of effort, touch pressure, and vibration if relevant) associated with this profession. A further relevant consequence of the effort applied during MIS is local muscle fatigue (LMF), an important factor to consider in musculoskeletal pathologies. The aim of this chapter is to present different methodological approaches by employing most advanced technologies to define the most appropriate posture that surgeons should adopt during MIS to decrease LMF apparition risk level and at the same time to increase capacity to variate the posture without reducing the precision task performance