Impact of Saliva Collection and Processing Methods on Aspartate Aminotransferase, Creatin Kinase and Lactate Dehydrogenase Activities

Abstract

We aimed to investigate the impact of saliva collection and processing methods on AST, CK and LDH. Saliva was collected from 17 healthy participants by a passive drool. Each saliva sample was distributed into 3 aliquots: not treated, centrifugued and passed through cotton. Centrifugation improved the precisión of assays and produced lower values of AST and CK. The use of cotton resulted in decreased levels of LDH. This stress the importance of the standaritzation of sample processing to measure enzymes in saliva