Ganchev, T.

English

Varna Medical University Press: Journals

Scripta Scientifica Medica, vol.28, Suppil.1, pp.231-232 Copyright ® Medical University, Varna E F F E C T S OF COLCHICINE ON ERYTHROPOIESIS AND ON SOME P R O P E R T I E S OF THE E R Y T H R O C Y T E S IN RATS T. Ganchev Department of Physiology, Varna It is known that assembling of cell tubulin molecules in microtu-bules is blocked by colchicine (CCH) (2,4). That disturbs mitotic pro-cesses and functions in many cell systems (2-4). Blood system at treatment with high doses of C C H reacts with leukocytosis, thrombo-cytosis and bone marrow aplasia. C C H depresses thrombocyte for : mation under "in vitro" (7), and "in vivo" conditions (5). There are no complex studies of C C H influence on erythropoiesis and on some properties of erythrocytes. Our aim was to investigate C C H influence on erythropoiesis and on some functions of mature erythrocytes. Ex-periments were carried out on 207 white female Wistar rats with 170-180 g body mass divided in three groups: 1 s t group (86) treated with C C H once daily - 0,5 mg/kg b.m. Colchicine (Merck, Germany); 2 group (26) treated with aamalumi-colchicine (Sigma, USA) once daily - 0,5mg/kg b.m. and 3 group (95) treated with the solvent for C C H and lumi-CCH (0,9 % NaCI) for control. Duration of the experiment was 3 days. Serum iron, Fe incorporation in newly formed erythro-cytes, erythrocytes' deformability and G-6-PD activity were deter-mined according to already described methods (1). 5 9 F e in newly-formed Er njnt,tjn Reticulocytes 28,85*4,48 8,6^3,06 X 31,17*-3,68 I I 7,44 % 72,12% p< 0,001 I Colchicine Lumi-Colchicine l p 1' 0.001 I Fig. 1 The data are presented as means ± SE towards 1000 erythr. PS Colchicine Lumi-Colchicine PS Fig.2 The data are presented as means ± SE 232 Determination of erythrocytes peroxide haemolysis was per­formed according to the method of Jager (8). Results are presented as means ± S E . Considerable decrease of reticulocytes with 72,12% (p< 0,001) and of 5 9 F e incorporation in newly formed ery­throcytes with 88,85% (p<0,001) (fig.1 and 2) in C C H treated rats was observed. Serum iron and coefficient of transferin saturation were increased (fig.3). Activity of G-6-PD in erythrocytes showed tendency for increase in com­parison with the control group. Deformability of erythrocytes and peroxide haemolysis after treatment with CCH did not show significant changes versus con­trol group. The probable reason for the depression of erythro­poiesis is antitubulin effect of C C H possibly on early erythroid cells and may be also reduced biogenesis of erythropoietin de­scribed by George and Rodgers (6). The reason for the increased serum iron according to us is its diminished utilization by ery­throid bone marrow, indicator for which is the considerable dec­rease of Fe incorporation by newly formed erythrocytes. It is obvious that the underlined de­pression of erythropoiesis by C C H does not influence substantially on the erythrocytic functions: deformability, peroxide resistance and activity of G-6-PD. R E F E R E N C E S : 1. Ганчев, Т. Epumpo- u тромбоцитопоеза и хормона лни и адренергично-медиирани влияния. Д о к т . дис. Вар­на, 1989. 2.Чалдъков, Г. Природа, 33, 1984, 28-34. 3. Chaldakov, G.H., V.N. Vankov. Atherosclerosis, 61 , 1986, 175-192. 4. Dustin, P. In: Microtubules. New York, Springer, 1978, 167-225. 5. Ganchev, Т., N. Negrev. Scr. Sci . Med., 26, 1989, 45-49. 6. George, W.J . , G.M. Rodgers. In: Kidney Hormones, v.2. Erythropoietin. J . Fisher, ed. London, Acad. Press, 1977, 329-359. 7. Handagama, P .J . , et al. Am-er. J . Veter. Res., 48, 1987, 1142-1146. 8. Jager, F.C. Nutr. Dieta (Basel), 10, 1968, 215-223. 36,26% [K 0,001 40,97 % P<0,10 Plasma iron Coefficient of TF saturation Colchicine Fig.3. Data are presented as per­cent deviation to control levels accepted as 0 

EFFECTS OF COLCHICINE ON ERYTHROPOIESIS AND ON SOME PROPERTIES OF THE ERYTHROCYTES IN RATS

https://journals.mu-varna.bg/index.php/ssm/article/download/3706/3172

EFFECTS OF COLCHICINE ON ERYTHROPOIESIS AND ON SOME PROPERTIES OF THE ERYTHROCYTES IN RATS

Abstract

Similar works

Full text

Available Versions

Varna Medical University Press: Journals