As bacteria are rapidly developing resistance against existing drugs, cannabinoids present a novel and exciting opportunity as a potential new source of antibiotics. Cannabinoid compounds have become an epicenter of research in recent years with numerous studies elucidating the therapeutic uses of a few of the numerous compounds such as Cannabidiol (CBD) and Cannabigerol (CBG). This study seeks to investigate the antimicrobial properties of these two aforementioned compounds on a number of gram-negative and gram-positive microbes such as Candida albicans, Streptococcus pyogenes and Pseudomonas aeruginosa. Using spread plating methods, various concentrations and mixtures of CBD and CBG were applied to selected microbes in order to observe the effects (if any) on colony formation. Initial results have indicated a strong effect on gram-positive organisms and little to no effect on gram-negative organisms. These early results corroborate published literature reports. Ongoing work includes an investigation into the lipopolysaccharide (LPS) layer found on the gram-negative bacteria. Through enzymatic treatment, LPS layer removal will be facilitated, and testing performed to determine the role this layer plays in the observed decreased antimicrobial activity of these organisms
