Pavlova, I.

Velikov, K.

English

Varna Medical University Press: Journals

16 CHANGES OF T H E W H I T E BLOOD PICTURE IN ANIMALS WITH SUBCHRONICAL MERCURY INTOXICATION AND PROTEIN HYDROLYSATE T R E A T M E N T K. Velikov, I . Pavlova Key-words: mercury intoxication — protein hydrolysate — white blood picture Our previous investigations have shown the favourable effect of protein hydrolysate upon certain haematological indexes of subchronical mersury into-xication in rats (1). T h i s propitious influence of protein hydrolysate on dama-ged parenchymatous organs was ascertained by other investigators, too (2, 3, 4) . The received results warrant us fcr studying the influence of protein hydrolysate on the white blood picture of subcronical mercury bichloride intoxication. Material and methods The study covers 85 male white rats wi th weight 150—200 g divided into groups: I s t — control; I I n d — injected w i t h , 0,25 mg/kg mercury bichloride s. c. for 45 days; I I I r d — injected w i t h 5 ml/kg protein hydrolysate i . m . for 45 days; I V t h — treated in the same way and period wi th mercury bichloride and protein hydrolysate. On the 7 t h , 15 t h , 30 t h and 45 t h day and 30 days after the treatment was over the leukocytes were counted up totally and differentially. Results and discussion The leukocyte count in a l l groups varies in normal ranges as compared wi th that of the controls. I t makes an impression that the leukocyte count diminishes on the 7 t h and 30 t h day after treatment w i t h mercury bichloride. Then it increa-ses in the recovery period. The differential blood picture demonstrates the following results: The band neutrophils increase statistically reliable on the 75 t h day in animals treated w i t h mercury bichloride (p<0,02) and diminished on the 45 t h day in those given pro-tein hydrolysate (p<0,02) . I n animals treated wi th both mercury bichloride and protein hydrolysate no statistical differences are found out. (f ig. 1). A diminution of the segmented neutrophils is observed in animals treated w i t h mercury bichloride in a l l investigated intervals. From the control value 2394 they decrease to 1261 on the 7 t h day and from 1536 — to 780 — on the 15 t h day. The data received on the 7 t h day are statist ically reliable (p<0,05) . There is a slight decrease in other intervals. I n the recovery period an equalization of segmented neutrophils' values is established in the 1 s t and 2 n d groups. I n animals treated w i t h protein hydrolysate alone or combined wi th mercury bichloride the values don't show any significant differences in a l l intervals as compared w i t h those of the controls (fig. 2) . Significant amplitudes of eosinophils about the control values are establi-shed from 54 to 27, on the 7 t h day and from 80 to 34, on the 15 t h day after treatment Changes of the white blood picture in. 49 with protein hydrolysate. On the 30 t h day there is a diminution in a l l groups studied: in the 2 n d — from 179 to 60; in the 3 r d — to 110, and in the 4 t h _ to 100. I n other intervals the eosinophils of experimental animals in the three groups ^ P v e r y near to the control values, ( f ig. 3) . Days of treatment controls mercury bichloride 0,25 my/ky protein hydrolysate prot. hydro!.+mere- bichloride 0,25my//eg i There is an unreliable decrease of monocytes in a l l groups on the 30 t h day. rom the control value of 147 they diminish to 63 in the 2 n d group, to 78 — in 3000 -Days of treatment controls —mercury bichloride 0,25my/ky protein hydro!ys ate prot hydro!. +merc. bichloride 0,25тд/кд ig. 2 the 3 r d one, and to 62 — in the 4 t h one. There is also an increase from the value f 93 to 206 in the animals treated w i t h protein hydrolysate on the 45 t h day which is stat ist ical ly significant (p<0,05) (f ig. 4 ) . 4 Scripta scienti i ica medica 50 К. Velikov, I . Pavlova The decrease of lymphocyte count in experimental animals on the 7 t h day in comparison to the control value isn't statistically reliable. These values are near to the control ones on the 15 t h , 30 t h and 45 t h day. The lymphocyte count in ­creases in the 2 n d group in the recovery period statistically significantly (p<0,001) (fig. 5 ) . 15 30 45 Days of- treatment -controls -mercury bichloride 0У25тд/кд 15 protein Hydrolysate —prot hiydrol.+merc. bichloride 0;25rr?g/kgr F i g . 3 280 240 > 200 % i 120 SO 40 О 15 30 45 Days of treatment controls mercury bichloride 0,25mg/kg 75 protein hydrolysate prot. hydroi.+ mere, bichloride 0,?5mg/kgr Fig . 4 Changes of the white blood picture in. 51 The experimental data show that the substances at these dosages used have led to variations of the white blood picture which are about the physiological norms. The lower rates of cells from the differential picture in experimental an i ­mals except those in the recovery period can be due to the action of these agents. The mercury bichloride suppresses the haemopoiesis at the dosages used. The influence of protein hydrolysate depends on its dose of alone and combined ap­plication with mercury bichloride. This dose was unsuccessfully determined and needs a correction in further investigations. К R E F E R E N C E S 1. В е л и к о в , К. и д р . (под печат). — 2 . М а л е в а, Е. , К. Д е м и р е в а . В : Юбил. науч. сес. ВМИ — Варна, I V . Варна, 1975, 1, 147—150. — З. П о п д и м и т -р о в, И., К. Д е м и р е в а . Експерим. мед. и морфол., 1975, З, 128—132. — 4. Р о р -d i m i t r o v , I . , К. D e m i r e v a . Arch. Union med. balkan., 1975, 5, 742—744. И З М Е Н Е Н И Я Б Е Л О Й К А Р Т И Н Ы КРОВИ У Ж И В О Т Н Ы Х В С Л Е Д С Т В И Е В В Е Д Е Н И Я Б Е Л К О В О Г О Г И Д Р О Л И З А Т А ПРИ С У Б Х Р О Н И Ч Е С К О Й И Н Т О К С И К А Ц И И ХЛОРИСТОЙ Р Т У Т Ь Ю К. Великое, И. Павлова Р Е З Ю М Е Эксперименты проведены на 85 белых крысах, распределенных в 4 группы: I группа — контрольная; I I группа — с подкожным введением хлористой ртути по 0,25 мг/кг; I I I груп­па — с интрамышечным введением белкового гидролизата по 5 мл/кг и I V группа — с одно­временным введением хлористой ртути и белкового гидролизата в указанных дозах. Указанные введения приводят к изменениям белой картины крови в границах физиоло­гической нормы. Белковый гидролизат, введенный самостоятельно и в сочетании с хлористой ртутью на оказывает значительного влияния на дифференциальную лейкоцитарную формулу при ртутной интоксикации. 

CHANGES OF THE WHITE BLOOD PICTURE IN ANIMALS WITH SUBCHRONICAL MERCURY INTOXICATION AND PROTEIN HYDROLYSATE TREATMENT

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CHANGES OF THE WHITE BLOOD PICTURE IN ANIMALS WITH SUBCHRONICAL MERCURY INTOXICATION AND PROTEIN HYDROLYSATE TREATMENT

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