Gardevska, V.

English

Varna Medical University Press: Journals

MORPHOLOGICAL CHANGES OF HUMAN EMBRYONAL K I D N E Y C E L L S I N F E C T E D WITH ECHO-VIRUSES V. Gardevska The cell-changes due to ECHO-viral infection are characterized by the productive type viral infectional changes. Numerous authors report similar disorders (1 , 2, 3, 4, 6, 7) which are related to chromatin-condension, peri-nuclear-zone lightening, nucleolar pycnosis, subcariolar migration of chro-matin and roughness of the nucleus which becomes semimoonlike (due to the impression of the developing cytoplasmatic component). The object of the present work is to investigate some early changes of the chromatin-level and especially of the nucleoli presuming the early diagnosis of ECHO-viral infections. Material and methods The study was carried out with primary trypsinized cell cultures of human embryonal kidney on lamellas. The infection was done by applying several types ECHO-viruses (1 , 9, 14, 19, 26) with a contact adaptation 30 min; 0.2 ml viral suspension with titer 10 3—10 6 . The lamellas were taken out on the 2 n d to 24 t h hour from the infection-beginning and figed in absolute alcohol for 10 min. The staining was done with hematoxilin and eosin, Romanovski—Giemsa and Mai Grunwald—Giemsa with and without a preliminary drying of the lamellas. Results and discussion The first and most early changes in the preparations stained with hema-toxilin-eosin were detected in the nucleus or nucleolus respectively; they were characterized by enlarged sizes of the nucleus and swelling and deformation of the nucleolus. The latter acquired multiangular form and very often were established thicker or thinner stripe or trabecule formations. The nucleo-lus-structure was grainy showing certain underlying of the basophylia here and there or small spotted lightenings (fig. 1). Some of the cases showed various structure — rodlike or canallike. Certain disorders of the nucleus-contours even if it was adhered to the cariollema was established (fig. 2). Chromatin preserved its predominant granular structure and at some points a relative roughness was seen. Further changes were an increased basophy-lia and nucleolus-rounding. The chromatin grains were like rays connected with the mass of the nucleolus where more pale vacuoles could be easier detected. Cytoplasmatic changes were also established and they formed the typical for ECHO-viruses incorporations. Later evolution of the cyto-pathical effect showed a decreased volume of the nucleoli and their location 120 V. Gardevska F ig . 2 near to the nuclear membrane. The preparations stained after Romanovski— Giemsa and Mai—Griinwald—Giemsa revealed out changes of the nucleus, specially the nucleolar associated chromatin. The nucleolus was stained clearly blue, while the nucleolar associated chromatin — intensively red. Too early, 2—3 hours after infection, even before the establishing of the swelling and deformation of the nucleoli, the nucleolar associated chromatin (red mass) was found near to the nucleolus itself (fig. 3). The structure of the nucleolus was nonhomogenous. The nucleoli were quite visible and with reduced sizes in the phase of forming the cytoplasmatic incorporation i ig. 4). Morphological changes of human embryonal. 121 The dynamics of the nucleolar changes after viral infection (ECHO-viruses) is most important for determination of the early cytopathic chan-Fig . 4 ges. The latter, being established only 3 hours after the beginning of the infection, prove the expressed reactivity of the nuclear apparatus and its participation in certain processes corresponding to the viral reproduction. I t is strongly recommended that a precise interpretation of the data must be done concerning the problem whether the changes are productive, hyper-N 122 Morphological changes of human embryonal. . . functional or degenerative. We consider our results as morphological disor­ders, but later nucleolar changes (condensation, hyperchromia and expres­sed vacuolization) can be refered to degenerative abnormalities. R Е F Е R E N C E S 1. А ц е в , С т . , С т . P а н г е л о в а, М. Н и к о л о в а . Върху развитието на цитоплазмените включвания при заразяване на клетъчни култури с вирус ЕСНО-19. — Епидемиол., микробиол, и инфекц. болести, 1967, № 4, 144. — 2. К о ­л е с н и к о в , А. В. Сравнительная характеристика цитопатических изменений в еднослойных культурах из тканей эмбрионов человека и амнистической ткани зара­женных кишечными вирусами. Вопр. вирусол., 1960, № 3, 328. — 3. П у х н е р , А. Ф. Сравнительное морфологическое изучение цитопатического действия в куль­туре клеток ткани почки и сердца обезьяны, инфициреванных вирусам полиомиелита, E C H O и коксаки. Вопр. вирусол., 1962, № 4, 74. — 4. Р а н г е л о в а, С т., С. А ц е в, Е. Ц и л к а. Вирусологични и сравнителни цитологични изследвания върху дина­миката на вирусно-клетъчното взаимодействие при някои ентеровируси. I . Конгрес бълг. микробиол. София, 1967 г. С , Б А Н , 711. — 5 . D a l e s , S. Penetration of animal viruses into cell. Progr. Med. V i r o l . , 7, 1965,1. — 6. G о d m a n, G . C. The cytopathology of enteroviral infection. Internal. Rev. Experim. Pathol. , 2, 1965, 67. — 7. S h a v e r , D . , N . , A . L . B a r r o n , D. Т. K a r s o n . Distinctive cvtopa-thology of E C H O viruses types 22 and 23. Proc. Soc. Experim. B i o l . , 106, 1961, 648. М О Р Ф О Л О Г И Ч Е С К И Е И З М Е Н Е Н И Я Я Д Р Ы Ш К А К Л Е Т О К Ч Е Л О В Е Ч Е С К О Й Э М Б Р И О Н А Л Ь Н О Й П О Ч К И , И Н Ф И Ц И Р О В А Н Н Ы Х Н Е К О Т О Р Ы М И В И Р У С А М И Г Р У П П Ы E C H O В. Гардевска Р Е З Ю М Е Исследованы ранние изменения хроматина ядрышек клеток человеческой эмбрио­нальной почки, инфицированных вирусами группы E C H O . Самые ранние изменения наблюдаются в течение второго — третьего часов после инфицирования. Они состоят увеличении размеров нуклеолярно ассоциированного хроматина, наблюдающегося в виде односторонне расположенной и соприкасающейся с ядрышком малиново-красной массы. Позже устанавливаются набухание и деформация ядрышек. Эти изменения могут быть использованы при диагностике ЕСНО-вирусных инфекций. 

MORPHOLOGICAL CHANGES OF HUMAN EMBRYONAL KIDNEY CELLS INFECTED WITH ECHO-VIRUSES

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MORPHOLOGICAL CHANGES OF HUMAN EMBRYONAL KIDNEY CELLS INFECTED WITH ECHO-VIRUSES

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