Belcheva, A.

Boshnakova, E.

Jelyazkov, D.

Mangarova, M.

Vassileva, L.

English

Varna Medical University Press: Journals

I N F L U E N C E OF UNITHIOL UPON NUCLEIFORMING OF BONE-MARROW LYMPHOCYTES OF RATS WITH SUBCHRONIC MERCURY INTOXICATION L. Vassileva, E. Boshnakova, A. Belcheva, M. Mangarova, D. Jelyazkov Our investigations on the changes of bone-marrow lymphocyte mitosis in rats wi th subchronic mercury intoxication (SMI) and the influence of U n i -thiol (U) upon this process (1) were continued in studying the nucleiforming cell processes showing their functional ac t iv i ty (3) . We could not find biblio-graphical data concerning the influence of antidotic therapy wi th U upon nuclei-forming in the conditions of S M I . We presume our study to be actual having in mind the established theratogenic effect of the organic mercury compounds (5, 6, 7, 8) , as wel l as the gonadotoxic and embryotoxic action of metal mer-cury vapours (2). Material and methods The study covers 90 white male rats divided into the following groups: 1) Control. 2) Treated s. d. w i th H g C l 2 (dose 0,25 mg/kg B W — V 3 0 D L 5 0 of the compound). 3) Treated i . m . wi th U (dose 0,38 mg/kg B W ) . 4) Treated wi th the combination H g C l 2 + U (same doses). The applied dose of U provides a double mollar ratio of the antidote to the injected dose of H g C l 2 . A l l animals are treated for a period of 45 days. 3 animals are ki l led every 7th, 1 5 t h , 3 0 t h and 4 5 t h day of treatment and another 3 animals — 30 days after the end of treatment. Smears of their femoral bone-marrow are prepared by using the method of F o x et a l . (4) . A total number of 40 000 blastic cells are studied: blastic cells without, wi th one and wi th more nuclei are counted; the number of the nuclei in 100 cells is determined; the results are analysed s tat is t ical ly by using the method of alternation. Results and discussion A decrease in number of blast cells in conditions of S M I is established. No changes in blast cells without nuclei are registered up to the 1 5 t h day ;after 3 0 t h day a slight decrease is established but from 4 5 t h day a further increase of their values is found. Blas t cells wi th nuclei show a constant number (com-pared to the controls) on 7 t h and 15 t h day, but on 3 0 t h day they tend to mul-t ip ly . After 4 5 t h day and during recreation their number goes down again. Constant number of a l l nuclei is registered un t i l 3 0 t h day, whereas after that a decrease is reported. We establish that the treating of animals wi th U tends to an increased number of blast cells in a l l intervals (except 7 t h day); the percent of blast cells without nuclei is unchanged. The latter are mult ipl ied on 1 5 t h , 3 0 t h and 4 5 t h 2 Scripta scientifica medica L . Vassileva, Е. Boshnakova, A. Belcheva, M MangarDva. day, whereas after recreation their number tends to that of the controls. U in­fluences upon the decrease in number of blast cells wi th nuclei as wel l as total number of a l l nuclei (100 cells) in any interval (except 7 t h day). The combination of H g C l 2 + U decreases unconsiderably the amount of a l l blast cells at the beginning of the experiment (up to 7 t h day), whi le in the rest intervals this effect is strongly considerable. There is a simultaneous in­crease of the percent of blast cells without nuclei , decrease in number of cells wi th nuclei , and also the total number of nuclei in 100 cells. H g C l 2 , applied alone, tends to get down the total number of blast cells in bone-marrow of rats wi th S M I . I t suppresses the processes of nucleiforming; theis effect is more expressed between 3 0 t h and 4 5 t h day and is s t i l l the same even after recreation. Our data correlate to those of V . M . Ignatiev (1980) who reports a stati­st ical ly reliable change in the functional state of rats' sperm cells and a de­crease in D N A - and RNA-amount in their testicles (established even in their first generation); the animals are subjected to a chronic intoxication wi th me­tal mercury vapours. I t is obvious that the application of U only tends to a percentage improvement of blast cells in experimental conditions and later recreation. However, it can not restore their functional ac t iv i ty ; this is because the number of cells without nuclei is higher, whereas that of the cell nuclei goes down (between 15 t h and 4 5 t h day of treatment). The combination of H g C l 2 + U makes stronger the suppressive effect of H g C l 2 over blastic ac t iv i ty and nucleiforming of bone-marrow cells of experi­mental animals. These data analysed together wi th our previous results (1) allow the conclusion that U , being antidote of H g C l 2 , provides unsufficient effect to normalize the affected by S M I functions and ac t iv i ty of bone-marrow cells of the experimental animals. • R E F E R E N C E S 1. В а с и л е в а , Л . , Е. Б о ш н а к о в а , А. Б е л ч е в а , М. М а н г ъ -р о в а , Д . Ж е л я з к о в . X V I Б а л к . Мед. Седм., Букурещ, I X , 1980. — 2. И г н а ­т ь е в , В. М. Гигиена и санитария, 3, 1980, 72—73. — 3. П р о к о ф ь е в а • Б е л -г о в с к а я , А. А. Основы цитогенетики человека. М., Медицина, 1969, 543. — 4. F о х, М., D. М. Z e i s s . Nature, 192, 1961, 1213. — 5. G a l e , Т. F . Environ­ment Research, 8, 1974, 2, 207—214. — 6. M е i - Q u е у, S., G . О k i t a. Toxic. Applic. Pharmacol., 38, 1976, 1, 207—216. — 7. O l s o n , F . С , E . Y . M a s s a r o . Toxic. Applic. Pharmacol., 3a, 1977, 2, 263—273. — 8. Y a m a g u c h i , S. , H . N u -n о t a n i . Environm. Physiol. Biochem., 4, 1974, 1, 7—15. Influence of unithiol upon nucleiforraing of bone-marrow.. 19 В Л И Я Н И Е У Н И Т И О Л А НА Я ДР Ы Ш КООБР АЗОВ АН И Е Л И М Ф О Ц И Т О В КОСТНОГО МОЗГА К Р Ы С ПРИ С У Б Х Р О Н И Ч Е С К О Й Р Т У Т Н О Й И Н Т О К С И К А Ц И И Л. Василева, Е. Бошнакова, А. Белчева, М. Мангарова, Д. Желязков Р Е З Ю М Е Исследовано действие унитиола на ядрышкообразование лимфоцитов костного мозга у крыс, которые подвергались субхронической ртутной интоксикации. Антидот исполь­зован в дозе, обеспечивающей его двойное молярное соотношение к введенному коли­честву хлорной ртути. Ядрышкообразование прослеживалось на 7-ой, 15-ый, 30-вый и 45-ый дни после его прекращения. Установлено, что комбинированное применение хлорной ртути (сулемы) и унитиола усиливает тормозящее действие хлорной ртути по отношению к бластной активности и ядрышкообразованию костномозговых клеток лабораторных животных. Высказывается мнение, что действие унитиола в качестве антидота хлорной ртути у крыс в условиях субхронической ртутной интоксикации не является достаточно эффективным для прео­доления нарушения ядрышкообразовательных процессов лимфоцитов костного мозга. 

INFLUENCE OF UNITHIOL UPON NUCLEIFORMING OF BONE-MARROW LYMPHOCYTES OF RATS WITH SUBCHRONIC MERCURY INTOXICATION

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INFLUENCE OF UNITHIOL UPON NUCLEIFORMING OF BONE-MARROW LYMPHOCYTES OF RATS WITH SUBCHRONIC MERCURY INTOXICATION

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