Arber JM

Atwood KN

Battifora H

Boenisch T

Carson FL

Harlow E

Packeisen J

Ramos-Vara JA

Rasmussen OF

Thrustfield M

True LD

Tubbs RR

Zeheb R

 This document is the consensus of the American Association of Veterinary Laboratory Diagnosticians (AAVLD) Subcommittee on Standardization of Immunohistochemistry on a set of guidelines for immunohistochemistry (IHC) testing in veterinary laboratories. Immunohistochemistry is a powerful ancillary methodology frequently used in many veterinary laboratories for both diagnostic and research purposes. However, neither standardization nor validation of IHC tests has been completely achieved in veterinary medicine. This document addresses both issues. Topics covered include antibody selection, fixation, antigen retrieval, antibody incubation, antibody dilutions, tissue and reagent controls, buffers, and detection systems. The validation of an IHC test is addressed for both infectious diseases and neoplastic processes. In addition, storage and handling of IHC reagents, interpretation, quality control and assurance, and troubleshooting are also discussed. Proper standardization and validation of IHC will improve the quality of diagnostics in veterinary laboratories. </jats:p

José A. Ramos-Vara

Matti Kiupel

Timothy Baszler

Laura Bliven

Bruce Brodersen

Brian Chelack

Keith West

Stefanie Czub

Fabio Del Piero

Sharon Dial

E. J. Ehrhart

Tanya Graham

Lisa Manning

Daniel Paulsen

Victor E. Valli

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Journal of Veterinary Diagnostic Investigation

Suggested Guidelines for Immunohistochemical Techniques in Veterinary Diagnostic Laboratories

Suggested Guidelines for Immunohistochemical Techniques in Veterinary Diagnostic Laboratories

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