Avcı, A.

Bülbül, U.

Fahrbach, M.

Litvinchuk, S.N.

Olgun, K.

Wielstra, B.

Üzüm, N.

Leiden University Scholary Publications

131CorrespondenceAvailable at http://www.salamandra-journal.com© 2019 Deutsche Gesellschaft für Herpetologie und Terrarienkunde e.V. (DGHT), Mannheim, GermanyCorrespondenceSALAMANDRA 55(2) 131–134 15 May 2019 ISSN 0036–3375Cracking cryptic species:  external characters to distinguish two recently recognized  banded newt species (Ommatotriton ophryticus and O. nesterovi)Nazan Üzüm1, Aziz Avcı1, Kurtuluş Olgun1, Ufuk Bülbül2, Michael Fahrbach3, Spartak N. Litvinchuk4 & Ben Wielstra5,61) Adnan Menderes University, Aydın, Turkey2) Karadeniz Technical University, Trabzon, Turkey3) Niedernhaller Str. 8/2, 74653 Criesbach, Germany4) Institute of Cytology, Russian Academy of Sciences, St. Petersburg, Russia5) Institute of Biology Leiden, Leiden University, Leiden, The Netherlands6) Naturalis Biodiversity Center, Leiden, The NetherlandsCorresponding author: Ben Wielstra, e-mail: ben.wielstra@naturalis.nlManuscript received: 5 October 2018Accepted: 9 February 2019 by Stefan LöttersCryptic species are species found to be genetically distinct, that previously went unrecognized due to their morpho-logical similarity (Beheregaray & Caccone 2007, Bick-ford et al. 2007, Pfenninger & Schwenk 2007). With the exponential increase in genetic data, many cryptic species have been uncovered. However, their status as cryptic spe-cies would only be temporary if diagnostic morphological differences, corresponding to the genetically defined spe-cies boundary, were subsequently to be found. This way the genetic revolution could stimulate a renewed interest in morphology (Wielstra & Arntzen 2016).Genetic studies in the family Salamandridae have regu-larly revealed cryptic species (e.g. Wielstra et al. 2013, Pa-bijan et al. 2017), with the most recent example provided by the banded newts (genus Ommatotriton) (Arntzen & Olgun 2000, Litvinchuk et al. 2005, Bülbül & Kutrup 2013, van Riemsdijk et al. 2017). A generally accepted split is Ommatotriton vittatus (Gray, 1835) versus O. ophryticus (Berthold, 1846). The latter has recently been further split into O. ophryticus sensu stricto (from here on O. ophryti­cus) and O. nesterovi (Litvinchuk, Zuiderwijk, Borkin & Rosanov, 2005). While morphological characters to dis-tinguish O. vittatus from the other two species have been described (Table 3 in Borkin et al. 2003), how to separate O. ophryticus from O. nesterovi is currently unclear.A superficial inspection of photographs taken in the field suggests two characters related to the ‘band’ in band-ed newts might be able to separate O. ophryticus from O. nestero vi (van Riemsdijk et al. 2018). In O. nesterovi, the lateral white band (I) appears to continue from the front limbs up to the eye and (II) appears to be disrupted by large specks on the tail (with a bluish hue in males), while this is not the case in O. ophryticus (Fig. 1). We conduct-ed a survey of museum specimens of known origin to test the reliability of these characters to distinguish between O. ophryticus and O. nesterovi.We surveyed adult specimens of O. nesterovi (n = 95 ♂♂; n = 160 ♀♀) and O. ophryticus (n = 181 ♂♂; n = 148 ♀♀) stored in the following museum collections: Zoological Museum of Adnan Menderes University (ZMADU), Aydın, Turkey; Karadeniz Technical University Zoology Research Laboratory (KZL), Trabzon, Turkey; Zoological Institute of the Russian Academy of Sciences, St. Petersburg (ZISP), Russia. Specimens could be unambiguously allocated to species based on their geographical origin, with O. nesterovi and O. ophryticus, as identified by genetic data, occurring west and east of the city of Samsun in Turkey (van Riems-dijk et al. 2017). For a detailed overview of material see the Supplementary material. For each specimen we determined whether the lateral white band continues from the front limbs up to the eye (Ia) or not (Ib), and whether the lateral white band is disrupted by large specks on the tail (IIa) or not (IIb). We also checked these characters in a less exhaus-tive survey of O. vittatus (n = 35 ♂♂; n = 73 ♀♀).132CorrespondenceResults are summarized in Table 1 and Figure 2 and a detailed overview of the results is in the Supplementary material. We find that for almost all O. nesterovi the lateral white band continues from the front limbs up to the eye (Ia: 96.9% of total, 91.6% of ♂♂ and 100.0% of ♀♀) and the lateral white band is disrupted by large specks on the tail (IIa: 98.0% of total, 98.9% of ♂♂ and 97.5% of ♀♀). On the other hand, for the majority of O. ophryticus individu-als the lateral white band does not continue from the front limbs up to the eye (Ib: 78.1% of total, 72.9% of ♂♂ and 84.5% of ♀♀) and the lateral white band is not disrupted by large specks on the tail (IIb: 90.3% of total, 90.6% of ♂♂ and 89.9% of ♀♀).We see a higher frequency of occurrence of typical O. nesterovi character states in O. ophryticus than the other way around and most (but not all) of these morphological deviations occur close to the contact zone (Fig. 2). When such deviations are observed, the character state is often less extremely expressed as in the species for which it is typical. When looking at both characters in combination, there is 1 (0.4%) O. nesterovi individual (1 [1.1%] ♂) that show both character states typical for O. ophryticus and there are 8 (2.4%) O. ophryticus individuals (5 [2.8%] ♂♂ and 2 [1.4%] ♀♀) that show both character states typical for O. nesterovi. Figure 1. A male and female representative of each banded newt species, genus Ommatotriton, for which vernacular names are in-troduced here. The inset shows known localities for the three species, with colours corresponding to species names (based on van Riemsdijk et al. 2017).Table 1. Survey of two ‘band characters’ in the three banded newt species, genus Ommatotriton. For each species it is noted for how many individuals (I) the lateral white band continues from the front limbs up to the eye (Ia) or not (Ib), and (II) the lateral white band is disrupted by large specks on the tail (IIa) or not (IIb).All Neck Tail ♂♂ Neck Tail ♀♀ Neck TailSpecies n Ia Ib IIa IIb n Ia Ib IIa IIb n Ia Ib IIa IIbO. nesterovi 255 247 8 250 5 95 87 8 94 1 160 160 0 156 4O. ophryticus 329 72 257 32 297 181 49 132 17 164 148 23 125 15 133O. vittatus 83 47 36 44 39 31 18 13 20 11 52 29 23 24 28133CorrespondenceFigure 2. Geographical survey for two ‘band’ characters dis-tinguishing two recently recognized banded newt species, ge-nus Ommatotriton: Panel (A) shows the O. nesterovi (blue) and O. ophryticus (red) localities studied for morphology (with spe-cies allocation based on genetic data; van Riemsdijk et al. 2017); note that localities within 25 kilometres of each other have been merged; details are in the Appendix; the black star represents the city of Samsun. Panel (B) shows whether I the band continues from the front limbs up to the eye (Ia, typical for O. nesterovi, blue pie slice) or not (Ib, O. ophtyricus, red). Panel (C) shows whether II the band is disrupted by large specks on the tail (IIa, typical for O. nesterovi, blue pie slice) or not (IIb, O. ophtyricus, red). Pie diameter reflects sample size. Results partitioned by sex are in the Appendix.Ommatotriton vittatus is easily distinguished from the other two species because the lateral white band is consid-erably broader (Fig. 1). The band only slightly more often than random extends from the front limbs towards the eye, as in O. nesterovi (although it is generally not so clearly de-marcated), and is also only slightly more than random dis-rupted by specks on the tail, as in O. nesterovi (although smaller and never bluish).We here studied the crypticness of the banded newts, a taxon that has had a turbulent taxonomical history, being formerly placed in a heavily polyphyletic Triturus as a sin-gle species, and later allocated to its own monotypic genus and split into first two and later three species (Arnt zen & Olgun 2000, Litvinchuk et al. 2005, Steinfartz et al. 2007, Bülbül & Kutrup 2013, van Riemsdijk et al. 2017). We present two external characters related to the ‘band’, (I) whether the band continues from the front limbs up to the eye or not and (II) whether the band is disrupted by large specks on the tail (with a blue hue in males) or not, that distinguish O. nesterovi and O. ophryticus (Figs 1–2).We do notice the occurrence of character states typical of one of the two species present in the other at low fre-quency and more often see typical O. nesterovi character states in O. ophryticus than the other way around (Table 1). The deviation mostly occurs close to the contact zone (Fig. 2). As O. ophryticus and O. nesterovi are known to be able to hybridize under artificial conditions (van Riems-dijk et al. 2018), the mixed morphological signal we find might indicate that hybridization between the two species occurs in nature as well. Similarly, anthropogenic trans-location might explain the localized occurrence of typi-cal O. nesterovi characteristics in the Russian part of the range of O. ophryticus (Fig. 2). However, it should be noted that there is currently no evidence from genetic data for admixture between the two species in their natural range (van Riemsdijk et al. 2017) and the morphological ‘devia-tion’ we observe might well concern minor intraspecific variation.Protecting biodiversity begins with recognizing it ex-ists, so accurate species delineation plays an important role in conservation biology. With the massive increase in the availability of genetic data, many new species have been discovered that had not been previously recognized from morphology (Beheregaray & Caccone 2007, Bickford et al. 2007, Pfenninger & Schwenk 2007). Guided by such genetic insights, a fresh look can be taken from the point of morphology and ‘cryptic species’ may turn out to not be so cryptic after all.AcknowledgementsIsolde van Riemsdijk provided help with the creation of Fig-ure 1. Photo credits for Figure 1: O. nesterovi by Max Sparre-boom (male) and Bayram Göçmen (female), O. ophryticus by Michael Fahrbach, and O. vittatus by Sergé Bogaerts. This project has received funding from the European Union’s Hori-zon 2020 research and innovation programme under the Marie Skłodowska-Curie grant agreement No. 655487.ReferencesArntzen, J. W. & K. Olgun (2000): Taxonomy of the banded newt, Triturus vittatus: morphological and allozyme data. – Amphibia-Reptilia, 21: 155–168.Beheregaray, L. & A. Caccone (2007): Cryptic biodiversity in a changing world. – Journal of Biology, 6: 9.Berthold, A. (1846): Mitteilung über das Vorkommen von Tri-tonen am Kaukasus. – Nachrichten von der Georg-Augusts-134CorrespondenceUniversität und der Königlichen Gesellschaft der Wissen-schaften zu Göttingen, 12: 188–190.Bickford, D., D. J. Lohman, N. S. Sodhi, P. K. L. Ng, R. Meier, K. Winker, K. K. Ingram & I. Das (2007): Cryptic species as a window on diversity and conservation. – Trends in Ecology & Evolution, 22: 148–155.Borkin, L. J., S. N. Litvinchuk & A. Zuiderwijk (2003): Band-molch, Triturus vittatus (Gray, 1835). – pp. 555–605 in: Gros-senbacher, K. & B. Thiesmeier (eds): Handbuch der Reptili-en und Amphibien Europas Bd 4/I. – Aula, Wiesbaden.Bülbül, U. & B. Kutrup (2013): Morphological and genetic vari-ations of Ommatotriton in Turkey. – Animal Biology, 63: 297–312.Gray. J. E. (1835): A manual of British vertebrate animals. – Pitt Press, Cambridge.Litvinchuk, S. N., A. Zuiderwijk, L. J. Borkin & J. Rosanov (2005): Taxonomic status of Triturus vittatus (Amphibia: Sala-mandridae) in western Turkey: trunk vertebrae count, genome size and allozyme data. – Amphibia-Reptilia, 26: 305–323.Pabijan, M., P. Zieliński, K. Dudek, M. Stuglik & W. Babik (2017): Isolation and gene flow in a speciation continuum in newts. – Molecular Phylogenetics and Evolution, 116: 1–12.Pfenninger, M. & K. Schwenk (2007): Cryptic animal species are homogeneously distributed among taxa and biogeographi-cal regions. – BMC Evolutionary Biology, 7: 121.Steinfartz, S., S. Vicario, J. W. Arntzen & A. Caccone (2007): A Bayesian approach on molecules and behavior: reconsider-ing phylogenetic and evolutionary patterns of the Salamandri-dae with emphasis on Triturus newts. – Journal of Experimen-tal Zoology Part B: Molecular and Developmental Evolution, 308B: 139–162.van Riemsdijk, I., J. W. Arntzen, S. Bogaerts, M. Franzen, S.N. Litvinchuk, K. Olgun & B. Wielstra (2017): The Near East as a cradle of biodiversity: a phylogeography of banded newts (genus Ommatotriton) reveals extensive inter-and in-traspecific genetic differentiation. – Molecular Phylogenetics and Evolution, 114: 73–81.van Riemsdijk, I., L. van Nieuwenhuize, I. Martínez-Solano, J. W. Arntzen & B. Wielstra (2018): Molecular data reveal the hybrid nature of an introduced population of banded newts (Ommatotriton) in Spain. – Conservation Genetics, 19: 249–254.Wielstra, B., A. B. Baird & J. W. Arntzen (2013): A multimar-ker phylogeography of crested newts (Triturus cristatus su-perspecies) reveals cryptic species. – Molecular Phylogenetics and Evolution, 67: 167–175.Wielstra, B. & J. W. Arntzen (2016): Description of a new spe-cies of crested newt, previously subsumed in Triturus ivan­bureschi (Amphibia: Caudata: Salamandridae). – Zootaxa, 4109: 73–80.Supplementary materialSurvey of two ‘band’ characters in the three banded newt species, genus Ommatotriton.

Cracking cryptic species: external characters to distinguish two recently recognized banded newt species (Ommatotriton ophryticus and O. nesterovi)

https://scholarlypublications.universiteitleiden.nl/access/item%3A2978769/download

Cracking cryptic species: external characters to distinguish two recently recognized banded newt species (Ommatotriton ophryticus and O. nesterovi)

Abstract

Similar works

Full text

Available Versions

Leiden University Scholary Publications