Bajema, B

Beeltje, Henry

de Weert, Jasperien

Hamers, Timo

Jonkers, Tim

Lamoree, MH

Schriks, Merijn

The goal of this study is to enhance the identification of unknown compounds inLC chromatograms of untreated raw water used for drinking water production by(1) preconcentration of samples and (2) prioritization of the unknown peaks basedon their bioactivity. Preconcentration was done by solid phase extraction (SPE) of3L large volume (LV) groundwater samples or by 6-week passive sampler (PS)deployment in the groundwater, using divinylbenzene as active phase. Bioactivityof the samples was tested as a decrease in bioluminescence in the Allivibriofischeri bioassay, which was chosen as a broad-scale bioanalytical tool respondingto many different types of pollutants. Samples were collected at three differentdrinking water production stations with low or high degree of anthropogenicinfluence. At two stations, different groundwater inlets were sampled. At onestation, samples were taken at different stages in the drinking water productionprocess. SPE and PS extracts were used for target analysis and non-targetscreening, and for testing in the bioassay before and after high-resolutionfractionation. More target compounds were detected in the concentrated LV andPS extracts than in a 1 mL direct injection of the water. As expected leastcompounds and lowest bioassay responses were detected in the raw water fromthe station with least anthropogenic influence. PS extracts gave much higherbioassay responses than the LV extracts. Both chemical and bioassay analysis ofsamples collected during subsequent steps in the drinking water productionprocess confirmed the efficient removal of (bioactive) contaminants. In addition tothe 4 peaks detected in the bioassay chromatogram of the reference station, themore anthropogenically influenced stations showed additional peaks indicative forthe presence of anthropogenic substances. Identification of suspect compoundsresponsible for these additional peaks will proceed according to a three-stepprocedure, i.e. based on (1) exact mass and isotopic pattern of compoundsavailable in different suspect lists, (2) fragment ions assigned to a precursoravailable in spectral libraries, and (3) estimated elemental composition. Referencestandards will be obtained for the suspect compounds to confirm their retentiontime and bioactivity. Results from the identification process are available by theend of May 2020, and will be presented at the SETAC Europe conference

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Detection and prioritization of unknown compounds in water extracts from drinking water production.

http://hdl.handle.net/1871.1/1089581a-31c7-46f8-bd8e-b1bc37cdba20

Detection and prioritization of unknown compounds in water extracts from drinking water production.

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