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Protein extraction protocols for optimal proteome measurement and arginine kinase quantitation from cricket Acheta domesticus for food safety assessment
Authors
Utpal Bose
James A. Broadbent
+9 more
Keren Byrne
Michelle L. Colgrave
Elecia B. Johnston
Angéla Juhász
Shaymaviswanathan Karnaneedi
Vachiranee Limviphuvadh
Andreas L. Lopata
Sebastian Maurer-Stroh
Sally Stockwell
Publication date
1 January 2021
Publisher
Edith Cowan University, Research Online, Perth, Western Australia
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Abstract
© 2021 Elsevier Ltd Insects have been consumed by people for millennia and have recently been proposed as a complementary, sustainable source of protein to feed the world\u27s growing population. Insects and crustaceans both belong to the arthropod family. Crustacean (shellfish) allergies are common and potentially severe; hence, the cross-reactivity of the immune system with insect proteins is a potential health concern. Herein, LC-MS/MS was used to explore the proteome of whole, roasted whole and roasted powdered cricket products. Eight protein extraction protocols were compared using the total number of protein and distinct peptide identifications. Within these data, 20 putative allergens were identified, of which three were arginine kinase (AK) proteoforms. Subsequently, a multiple reaction monitoring MS assay was developed for the AK proteoforms and applied to a subset of extracts. This targeted assay demonstrated that allergen abundance/detectability varies according to the extraction method as well as the food processing method
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