Acknowledgments This work was funded in part by the William and Melinda Gates Foundation, award 42917 to JPN and OCS; US National Institutes of Health grants 5R01HG005220 to HCB, 5R01HG004885 to MP; US National Science Foundation Graduate Research Fellowship award DGE0750616 to JNP; AWW and JP are funded by The Wellcome Trust (Grant No. WT098051).Peer reviewedPublisher PD

Adeyemi, Mitchell

Ahmed, Dilruba

Ahmed, Firoz

Alam, Meer

Amin, Ruhul

Antonio, Martin

Astrovskaya, Irina

Bravo, Hector

Breiman, Robert F

Ebruke, Chinelo

Hossain, M Anowar

Ikumapayi, Usman N

Juma, Jane

Kotloff, Karen

Levine, Myron M

Lindsay, Brianna

Mai, Volker

Mailu, Euince

Morris, J Glenn

Nataro, James P

Ochieng, John B

Omore, Richard

Ouma, Emmanuel

Oundo, Joseph

Panchalingam, Sandra

Parkhill, Julian

Paulson, Joseph

Pop, Mihai

Rance, Richard

Saha, Debasish

Siddiqui, Sabbir

Stares, Mark

Stine, O Colin

Tamboura, Boubou

Walker, Alan W

English

PubMed

Diarrheal diseases continue to contribute significantly to morbidity and mortality in infants and young children in developing countries. There is an urgent need to better understand the contributions of novel, potentially uncultured, diarrheal pathogens to severe diarrheal disease, as well as distortions in normal gut microbiota composition that might facilitate severe disease. We use high throughput 16S rRNA gene sequencing to compare fecal microbiota composition in children under five years of age who have been diagnosed with moderate to severe diarrhea (MSD) with the microbiota from diarrhea-free controls. Our study includes 992 children from four low-income countries in West and East Africa, and Southeast Asia. Known pathogens, as well as bacteria currently not considered as important diarrhea-causing pathogens, are positively associated with MSD, and these include Escherichia/Shigella, and Granulicatella species, and Streptococcus mitis/pneumoniae groups. In both cases and controls, there tend to be distinct negative correlations between facultative anaerobic lineages and obligate anaerobic lineages. Overall genus-level microbiota composition exhibit a shift in controls from low to high levels of Prevotella and in MSD cases from high to low levels of Escherichia/Shigella in younger versus older children; however, there was significant variation among many genera by both site and age. Our findings expand the current understanding of microbiota-associated diarrhea pathogenicity in young children from developing countries. Our findings are necessarily based on correlative analyses and must be further validated through epidemiological and molecular techniques.https://doi.org/10.1186/gb-2014-15-6-r7

Bravo, Hector Corrada

Alam, Meer Taifur

Digital Repository at the University of Maryland

Diarrhea in young children from low-income countries leads to large-scale alterations in intestinal microbiota composition

Acknowledgments This work was funded in part by the William and Melinda Gates Foundation, award 42917 to JPN and OCS; US National Institutes of Health grants 5R01HG005220 to HCB, 5R01HG004885 to MP; US National Science Foundation Graduate Research Fellowship award DGE0750616 to JNP; AWW and JP are funded by The Wellcome Trust (Grant No. WT098051).Peer reviewe

Aberdeen University Research

BACKGROUND: Diarrheal diseases continue to contribute significantly to morbidity and mortality in infants and young children in developing countries. There is an urgent need to better understand the contributions of novel, potentially uncultured, diarrheal pathogens to severe diarrheal disease, as well as distortions in normal gut microbiota composition that might facilitate severe disease. RESULTS: We use high throughput 16S rRNA gene sequencing to compare fecal microbiota composition in children under five years of age who have been diagnosed with moderate to severe diarrhea (MSD) with the microbiota from diarrhea-free controls. Our study includes 992 children from four low-income countries in West and East Africa, and Southeast Asia. Known pathogens, as well as bacteria currently not considered as important diarrhea-causing pathogens, are positively associated with MSD, and these include Escherichia/Shigella, and Granulicatella species, and Streptococcus mitis/pneumoniae groups. In both cases and controls, there tend to be distinct negative correlations between facultative anaerobic lineages and obligate anaerobic lineages. Overall genus-level microbiota composition exhibit a shift in controls from low to high levels of Prevotella and in MSD cases from high to low levels of Escherichia/Shigella in younger versus older children; however, there was significant variation among many genera by both site and age. CONCLUSIONS: Our findings expand the current understanding of microbiota-associated diarrhea pathogenicity in young children from developing countries. Our findings are necessarily based on correlative analyses and must be further validated through epidemiological and molecular techniques

Corrada Bravo, Hector

LSHTM Research Online

Diarrhea in young children from low-income countries leads to large-scale alterations in intestinal microbiota composition.

Mihai Pop

Alan W Walker

Joseph Paulson

Brianna Lindsay

Martin Antonio

M Hossain

Joseph Oundo

Boubou Tamboura

Volker Mai

Irina Astrovskaya

Hector Bravo

Richard Rance

Mark Stares

Myron M Levine

Sandra Panchalingam

Karen Kotloff

Usman N Ikumapayi

Chinelo Ebruke

Mitchell Adeyemi

Dilruba Ahmed

Firoz Ahmed

Meer Alam

Ruhul Amin

Sabbir Siddiqui

John B Ochieng

Emmanuel Ouma

Jane Juma

Euince Mailu

Richard Omore

J Morris

Robert F Breiman

Debasish Saha

Julian Parkhill

James P Nataro

O Stine

Springer - Publisher Connector

Background: Diarrheal diseases continue to contribute significantly to morbidity and mortality in infants and young children in developing countries. There is an urgent need to better understand the contributions of novel, potentially uncultured, diarrheal pathogens to severe diarrheal disease, as well as distortions in normal gut microbiota composition that might facilitate severe disease. Results: We use high throughput 16S rRNA gene sequencing to compare fecal microbiota composition in children under five years of age who have been diagnosed with moderate to severe diarrhea (MSD) with the microbiota from diarrhea-free controls. Our study includes 992 children from four low-income countries in West and East Africa, and Southeast Asia. Known pathogens, as well as bacteria currently not considered as important diarrhea-causing pathogens, are positively associated with MSD, and these include Escherichia/Shigella, and Granulicatella species, and Streptococcus mitis/pneumoniae groups. In both cases and controls, there tend to be distinct negative correlations between facultative anaerobic lineages and obligate anaerobic lineages. Overall genus-level microbiota composition exhibit a shift in controls from low to high levels of Prevotella and in MSD cases from high to low levels of Escherichia/Shigella in younger versus older children; however, there was significant variation among many genera by both site and age. Conclusions: Our findings expand the current understanding of microbiota-associated diarrhea pathogenicity in young children from developing countries. Our findings are necessarily based on correlative analyses and must be further validated through epidemiological and molecular techniques.12 page(s

Morris, J. Glenn

Stine, O. Colin

Hossain, M. Anowar

Macquarie University ResearchOnline

Crossref

Genome Biology

https://genomebiology.biomedcentral.com/track/pdf/10.1186/gb-2014-15-6-r76?site=genomebiology.biomedcentral.com

Diarrhea in young children from low-income countries leads to large-scale alterations in intestinal microbiota composition

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