The composition of the essential oil produced from aerial parts of Salvia montbretii Benth.(Lamiaceae), was analyzed by GC and GC-MS. Forty-six compounds constituting about 97.7% of the essential oil were characterized.The main compounds were characterized as ?-caryophyllene (32.8%), ?-pinene (9.8%), ?-humulene (8.2%), 12-hydroxy-?-caryophyllene acetate (6.6%), germacrene D (4.9%) and ?-pinene (4.5%

Abak, Fatma

Atamov, Vagif

Kürkçüoğlu, Mine

Yıldız, Gülsüm

Records of Natural Products

English

DSpace @ Recep Tayyip Erdoğan Üniversitesi Akademik Açık Arşiv

    ORIGINAL ARTICLE   The article was published by ACG Publications www.acgpubs.org/RNP © September-October/ 2018 EISSN:1307-6167 DOI: http://doi.org/10.25135/rnp.51.17.12.080    Rec. Nat. Prod. 12:5 (2018) 426-431   Composition of the Essential Oil of Salvia montbretii Benth. from Turkey  Fatma Abak1, Gulsum Yildiz2, Vagif Atamov1and Mine Kurkcuoglu2*  1Recep Tayyip Erdoğan Unıversity, , Faculty of Science and Literature, Department of Biology,  Rize, Türkiye 2Anadolu University, Faculty of Pharmacy, Department of Pharmacognosy, 26470, Eskisehir, Türkiye   (Received December 27, 2017; Revised January 28, 2018; Accepted January 30, 2018)  Abstract: The composition of the essential oil produced from aerial parts of Salvia montbretii Benth.(Lamiaceae), was analyzed by GC and GC-MS. Forty-six compounds constituting about 97.7% of the essential oil were characterized.The main compounds were characterized as -caryophyllene (32.8%), -pinene (9.8%), -humulene (8.2%), 12-hydroxy--caryophyllene acetate (6.6%), germacrene D (4.9%) and -pinene (4.5%).  Keywords: Salvia montbretii Benth; oil composition; -caryophyllene; -humulene; -pinene. © 2018 ACG Publications. All rights reserved.   1. Introduction  Salvia L. is one of the largest genera in the family Lamiaceae with 900 species in the World. Salvia genus is represented by 100 species, 53% of whic are endemic in Turkey [1-3]. Salvia montbretii is named “ kara salba” in South-East Anatolia, Turkey.  Numerous Salvia species are used both internally and externally as folk medicines all around the World. There are several reports on the medicinal uses of some species of the genus, mainly against cold, skin infections, wounds, pharyngitis, stomatitis, stomachache, headache, memory enhancement and galactorrhoea [4]. The chemical compositions of several Salvia species have been extensively investigated revealing the presence of terpenes, triterpenoids, diterpenoids, tannins, phenolics and essential oil [3-18]. Previous phytochemical studies on Salvia montbretii Benth. have reported the presence of abiatane diterpenes and triterpenoids. Salvibretol, 1-oxosalvibretol, 6-hydroxysalvinolone, 7-hydroxytaxodione, 7,7´-bistaxodione, and 11,´-didehydroxy-7,7´-dihydroxytaxodione, montbretol, montbretyl 12-methyl ester, 14-hydroxy ferruginol, 3--O-cis-p-coumaroylmonogynol and 3--O-trans-p-coumaroylmonogynol were isolated from root of S. montbretii [15-17].                                                   * Corresponding author: E-Mail: minekurkcuoglu@gmail.com  Phone:+90 222 335 05 80    Guo et.al., Rec. Nat. Prod. (2018) 12:5 426-431   427   In previous study, water-distilled essential oil of S. montbretii was analyzed using GC and GC–MS and thymol (24.0%) and caryophyllene oxide (26.9%) were found to be the main components in the oil [19]. 2. Materials and Methods  2.1. The Plant Material   Salvia montbretii was collected from Şanlıurfa: between Çatak –Bozova in Turkey on 20 May 2017. The voucher specimenhas been deposited at the Herbarium in the  Recep Tayyip Erdoğan Unıversity (RTEUB 6079), Rize, Turkey (Voucher specimen no: FABAK 1478). The plant material was identified by Prof. Dr. Vagif ATAMOV (Recep Tayyip Erdoğan University, Faculty of Science and Literature, Department of Biology , Rize , Turkey).  2.2. Isolation of the Essential Oil Aerial parts of the plant were water distilled for 3 h using a Clevenger-type apparatus.The essential oil was stored at 4°C in the dark until analyzed. Oil yield in the sample was less than 0.1%.  2.3. Gas Chromatography (GC) and Gas Chromatography -Mass Spectrometry (GC-MS) Analysis The oil was analyzed by Gas Chromatography (GC) and Gas Chromatography-Mass Spectrometry (GC-MS) using a Agilent GC-MSD system (Mass Selective Dedector-MSD).   2.3.1. GC-MS Analysis   The GC-MS analysis was carried out with an Agilent 5975 GC-MSD system (Agilent, USA; SEM Ltd., Istanbul, Turkey). Innowax FSC column (60m × 0.25mm, 0.25m film thickness) was used with helium as carrier gas (0.8 mL/min). GC oven temperature was kept at 60C for 10 min and programmed to 220C at a rate of 4C/min, and kept constant at 220C for 10 min and then programmed to 240C at a rate of 1C/min.  Split ratio was adjusted 40:1. The injector temperature was at 250C. The interphase temperature was at 280C. MS were taken at 70 eV. Mass range was from m/z 35 to 450.   2.3.2. GC Analysis GC analyses were performed using an Agilent 6890N GC system. FID temperature was set to 300C and the same operational conditions were applied to a triplicate of the same column used in GC–MS analyses. Simultaneous auto injection was done to obtain equivalent retention times. Relative percentages of the separated compounds were calculated from integration of the peak areas in the GC-FID chromatograms.  2.4. Identification of Compounds  The components of essential oils were identified by comparison of their mass spectra with those in the Baser Library of Essential Oil Constituents, Adams Library, MassFinder Library, Wiley GC/MS Library [20-22] and confirmed by comparison of their retention indices. These identifications were accomplished by comparison of retention times with authentic samples or by comparison of their relative retention index (RRI) to a series of n-alkanes. Alkanes were used as reference points in the calculation of relative retention indices (RRI) [23]. Relative percentage amounts of the separated compounds were calculated from FID chromatograms. The analysis results are expressed as mean percentage ± standard deviation (SD) (n = 3) as listed in Table 1.     Essential oil of Salvia montbretii Benth.  428   3.  Results and Discussion   Essential oil of of Salvia montbretii was hydrodistilled from aerial parts for 3 h using a Clevenger apparatus and analysed by gas chromatography (GC), and gas chromatography-mass spectrometry (GC-MS).                     Table 1. Composition of the essential oil of Salvia montbretii Benth. RRIa RRIb Compounds % ID 1025c 1032 -Pinene 4.5± 0.1 tR, Ms 1117c 1118 -Pinene 9.8± 0.3 tR, Ms 1122c 1132 Sabinene 1.6± 0.2 tR, Ms 1160c 1174 Myrcene 1.4± 0.0 tR, Ms 1212c 1203 Limonene 2.7± 0.1 tR, Ms 1213c 1213 1,8-Cineole 1.4± 0.1 tR, Ms 1232c 1246 (Z)--Ocimene 0.2± 0.0 tR, Ms 1245c 1255 -Terpinene 2.9± 0.1 tR, Ms 1249d 1266 ( E)--Ocimene 0.5± 0.1 tR, Ms 1282c 1280 p-Cymene 0.5± 0.1 tR, Ms 1282c 1290 Terpinolene 0.2± 0.0 tR, Ms 1391d 1400 Nonanal 0.2± 0.0 Ms 1399d 1406 -Fenchone 0.2± 0.0 Ms 1414d 1424 Hexyl butyrate 0.1± 0.0 Ms 1443f 1444 Dimethyl tetradecane 0.3± 0.0 Ms 1468d 1479 -Elemene 0.3± 0.0 Ms 1487d 1484 Bicycloelemene 0.4± 0.1 Ms 1488c 1497 -Copaene 0.7± 0.1 Ms 1496d 1506 Decanal 0.3± 0.1 Ms 1523c 1535 -Bourbonene 0.8± 0.0 tR, Ms 1543c 1553 Linalool 0.3± 0.0 tR, Ms 1554d 1565 Linalyl acetate 1.2± 0.1 tR, Ms 1576c 1577 -Ylangene     0.5± 0.0 Ms 1591d 1600 -Elemene 0.5± 0.0 Ms 1600g 1600 Hexadecane tR, Ms 1598d 1612 -Caryophyllene 32.8± 0.1 tR, Ms 1602d 1617 Lavandulyl acetate 0.2± 0.1 Ms 1639d 1651 -Elemene 1.9± 0.1 Ms    Guo et.al., Rec. Nat. Prod. (2018) 12:5 426-431   429                                             RRIa: RRI from literature (c [18]; d [25] ; e [26] ; f [27] ; g [28]) for polar column values; RRIb: RRI Relative retention indices experimentally calculated against n-alkanes; *: correct isomer not identified %: calculated from the FID chromatograms and expressed as mean ± SD (n = 3); t: Trace (<0.1 %); ID: Identification Method; Identification method based on the relative retention indices (tR) of authentic compounds on a HP Innowax column; Ms, identified on the basis of computer matching of the mass spectra with those of the in-house Baser Library of Essential Oil Constituents, Adams, MassFinder  and Wiley libraries.  Forty-six compounds constituting about 97.7% of the essential oil were characterized. The main compounds were characterized as -caryophyllene (32.8%), -pinene (9.8%), -humulene (8.2%), 12-hydroxy--caryophyllene acetate  (6.6%), germacrene D (4.9%) and -pinene (4.5%). The analysis results are given in Table 1. 1651d 1668 (Z)--Farnesene 2.4± 0.0 Ms 1663c 1687 -Humulene 8.2± 0.0 tR, Ms able 1 Continued…   1700f 1700 Heptadecane 0.8± 0.0 tR, Ms 1708c 1726 Germacrene D 4.9± 0.0 Ms 1718d 1733 Neryl acetate 0.2± 0.0 Ms 1800g 1800 Octadecane  0.6± 0.1 tR, Ms 1935d 1957 ( E)--Ionone  t Ms 1986d 2008 Caryophyllene oxide  2.3± 0.1 tR, Ms 2124c 2131 Hexahydrofarnesyl acetone 1.3± 0.1 tR, Ms 2126c 2144 Spathulenol t tR, Ms 2164d 2205 Thymol 1.1± 0.1 tR, Ms 2211d 2246 Carvacrol  t tR, Ms 2331e 2316 12-Hydroxy--caryophyllene acetate 6.6± 0.0 Ms 2392d 2392 Caryophyllenol II 0.5± 0.1 Ms 2571c 2622 Phytol 0.7± 0.0 Ms 2628d 2676 Manool 0.3± 0.0 Ms 2687d 2696 Tetradecanoic acid 0.6± 0.1 tR, Ms 2913d 2931 Hexadecanoic acid  0.8± 0.3 Ms   Grouped compounds (%)     Monoterpene hydrocarbones  24.1    Oxygenated monoterpenes  3.5    Sesquiterpenes hydrocarbones  53.4    Oxygenated sesquiterpenes  2.8    Others  13.9    Total %  97.7     Essential oil of Salvia montbretii Benth.  430 However, according to Ozen et al., the main compounds of S. montbretii collected from South-eastern Turkey in June 2002 (DUF 9473; Diyarbakır: between Silvan – Batman), were reported  thymol (24.0%) and caryophyllene oxide (26.9%) [19]. The sesquiterpene hydrocarbon -caryophyllene is present in almost all of the Salvia species. -caryophyllene; S. aramiensis Rech. f. (7.55%), S. atropatana Bunge  (1.39%), S.aucheri Bentham var. aucheri (3.02%), S. bracteata Banks & Sol. (1.36%), S. caespitosa Montbret& Aucher ex Benth. (1.44%), S. kronenburgii Rech. f. (10.27%), S. modesta Boiss. (3.78%), S. multicaulis Vahl (2.09%), S. pachystachys Trautv (4.83%), S. pisidica Boiss. & Heldr. (1.53%), S. potentillifolia Boiss. Heldr. ex Benth. (1.09%) S. rosifolia Sm. (1.45%) and S. sclarea L. (1.05%) [3]. Salvia montbretii essential oil has high sesquiterpenes hydrocarbones content (53.4%). To the best of our knowledge, this is the first report on the GC and GC-MS determination of the essential oil composition of S. montbretii in this locality. These results provide further support to earlier observations on the existence of distinct chemotypes [3, 24] of the same species of Salvia naturally growing the same area.  Disclosure Statement No potential conflict of interest was reported by the authors  ORCID   Fatma Aabak: 0000-0002-9653-1679  Gülsüm Yıldız: 0000-0002-1697-3793  Vagif Atamov: 0000-0002-6718-7979  Mine Kurkcuoglu:0000-0002-9375-0294        References  [1]     I. C. Hedge (1982). Salvia L. In: P.H. (ed.) Flora of Turkey and the East Aegean Islands. Vol.7. Edinburgh Univ. Press, Edinburgh. pp. 400-461. [2]    A. Güner, S. Aslan, T. Ekim, M. Vural, M. T. Babac (2012). List of Turkish Flora (Vascular Plants). Nezahat Gökyigit Botanik Bahcesi ve Flora Arastırmaları Dernegi Yayını. Istanbul, 578. [3]    S. D. Hatipoglu, N. Zorlu, T. Dirmenci, A. C. Goren, T. Ozturk and G. Topcu (2016). Determination of volatile organic compounds in fourty five Salvia Species by thermal desorption-GC-MS technique, Rec. Nat. Prod. 10:6, 659-700. [4]     G. G. Toplan, M. Kurkcuoglu, F. Goger, G. İscan, H. G. Agalar, A. Mat, K. H. C. Baser, M. Koyuncu and G. Sarıyar (2017). 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Composition of the essential oil of Salvia montbretii Benth. from Turkey

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Composition of the essential oil of Salvia montbretii Benth. from Turkey

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