Not AvailableThe food safety and quality control in the seafood processing industry necessitates rapid detection of pathogens. Most of the pathogens occur in the food in small numbers only so that detection becomes very difficult. Some of the pathogens like Vibrio cholerae also exist in the so called "viable but non-culturable" state in the environment so that ordinary biochemical protocol becomes unsuccessful to detect their presence. Molecular methods such as polymerase chain reaction (PCR) has the advantage over the existing biochemical detection method in detection very small numbers of stressed cells of these pathogens and that too in a relatively short time. A PCR method is described to detect Vibrio cholerae in fish by amplifying a 564 bp fragment of the CTX gene.Not Availabl
