One hundred forty-nine strains of bacteria were isolated from 24 powder-puffs used daily by 24 female students. All the powder-puffs had some bacteria which grew on Soybean-Casein-Digest-Lecithin-Polysorbate 80 broth agar medium. Six powder-puffs had many bacteria of more than 1000 colony forming unit per 1 ml of powder-puff washing. Bacteria including genus Staphylococcus, genus Micrococcus and genus Bacillus were isolated, of which common species were S. epidermidis and B.subtilis or B.pumilus. The incidence of S.aureus was as low as 7 strains in 149 ones
