Methyl jasmonate (MJ) and cis jasmone (CJ), lipid-derived plant stress hormones, were studied for their effects against both hormone dependent (LNCaP) and hormone independent (PC3) human prostate cancer cell lines. Both 2 mM MJ or CJ inhibited the growth of the cell lines. An alamar blue assay was used to determine IC50 values for MJ and CJ in both cell lines at 24 hrs, 48 hrs and 72 hrs. At 24 hrs the MJ IC50 values were 2.25 and 2.05 while the CJ values were 3.00 and 1.25 in the PC3 and LNCaP lines respectively. Cell cycle analysis revealed that MJ and CJ induced apoptosis in both cell lines as well as activated caspase-3. An in vitro assay showed that MJ and CJ did not significantly inhibit either 5-lipoxygenase or 15-lipoxygenase. 5-HETE was able to stimulate cell growth in the presence or absence of MJ in both the cell lines
